Enhancing the practicability of the method for proteome analysis based on a new isolation scheme of C-terminal peptides.

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We have established a method to identify proteins by MALDI mass spectrometry of the C-terminal peptide obtained from a protein in which all the carboxyl groups were protected by exhaustive amidation. The optimized protocol to concentrate or isolate the C-terminal peptide from the proteolytic digest of the fully amidated protein includes the use of a strong anion-exchange HPLC column to remove anionic peptides.