Enhancing the practicability of the method for proteome analysis based on a new isolation scheme of C-terminal peptides.

增强基于C端肽新分离方案的蛋白质组分析方法的实用性。

• 批准号: 21510225
• 负责人: NAKAZAWA Takashi
• 金额: $ 3万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21510225/
• 关键词: タンパク質; C末端ペプチド; プロテオミクス; アミノ酸配列解析; 質量分析; 化学修飾; 安定同位体標識; 強塩基性陰イオン交換体; ペプチドの分離; イオン交換クロマトグラフィー; 文化財科学

We have established a method to identify proteins by MALDI mass spectrometry of the C-terminal peptide obtained from a protein in which all the carboxyl groups were protected by exhaustive amidation. The optimized protocol to concentrate or isolate the C-terminal peptide from the proteolytic digest of the fully amidated protein includes the use of a strong anion-exchange HPLC column to remove anionic peptides.

我们已经建立了一种方法来识别蛋白质的MALDI质谱的C-末端肽从蛋白质中，其中所有的羧基被保护的穷举酰胺化。从完全酰胺化蛋白质的蛋白水解消化物中浓缩或分离C-末端肽的优化方案包括使用强阴离子交换HPLC柱以除去阴离子肽。

项目成果

タンパク質又はペプチドのC末端を修飾する方法

修饰蛋白质或肽的C末端的方法

• 发表时间: 2004

C-Terminal sequencing of proteins by MALDI mass spectrometry through the specific derivatization of theα-carboxyl group with 3-aminopropyltris(2, 4, 6-trimethoxyphenyl) phosphonium bromide

通过用 3-氨基丙基三(2,4,6-三甲氧基苯基)溴化鏻对 α-羧基进行特异性衍生化，通过 MALDI 质谱法对蛋白质进行 C 末端测序

• 期刊: Anal. Bioanal. Chem
• 作者: Chihiro Nakajima;Hiroki Kuyama;Takashi Nakazawa;Osamu Nishimura
• 通讯作者: Osamu Nishimura

A new approach for detecting C-terminal amidation of proteins and peptides by mass spectrometry in conjunction with chemical derivatization

• DOI: 10.1002/pmic.200900267
• 发表时间: 2009-08-01
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Kuyama, Hiroki;Nakajima, Chihiro;Tsunasawa, Susumu
• 通讯作者: Tsunasawa, Susumu

The triple helical structure and stability of collagen model peptide with 4(s)-hydroxyprolyl-pro-gly units

• DOI: 10.1002/bip.21730
• 发表时间: 2012-01-01
• 期刊: BIOPOLYMERS
• 影响因子: 2.9
• 作者: Motooka, Daisuke;Kawahara, Kazuki;Kobayashi, Yuji
• 通讯作者: Kobayashi, Yuji

A method for N-terminal de novo sequencing of N^α-blocked proteins by mass spectrometry

一种利用质谱法对 N^α 封闭蛋白进行 N 端从头测序的方法

• DOI: 10.1039/c0an00384k
• 发表时间: 2011
• 期刊: Analyst
• 影响因子: 4.2
• 作者: Chihiro Nakajima;Hiroki Kuyama;Takashi Nakazawa;Osamu Nishimura;and Susumu Tsunasawa
• 通讯作者: and Susumu Tsunasawa