Basic studies on metabolic versatility of plant natural products and biosynthetic engineering with leguminous model systems

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To clarify the mechanism that creates the vast structural diversity of plant natural products, cDNAs encoding the enzymes involved in the biosynthesis of flavonols of a leguminous model plant Lotus japonicus, a specific triterpenoid of Aster tataricus and polyphenols of Acacia mangium were cloned, and the enzyme reactions were analyzed. Also, metabolic engineering was attempted with L. japonicus hairy root systems in which the expression of genes of flavonoid pathway was altered. Furthermore, the phytoalexin response of the cultured L. japonicus cells against various microbe signals was analyzed in order to facilitate the engineering efficiencies.