A study on new approaches in soft x-ray microscopy of living cells in the spectral region of "carbon window"

在“碳窗口”光谱区的软X射线显微镜被认为是观察活细胞的一种方法。在软X射线显微镜上安装了一种新开发的使用液氮和氧气气体的低温样品系统，能够进行“碳窗”观测。利用该显微镜，验证了聚苯乙烯乳胶球在低温下的辐射损伤减轻。我们还成功地在低温下对生物有机体进行了实验观察。此外，碲对耐辐射球菌的图像对比度增强产生了影响，耐辐射球菌是在“碳窗口”观察活细胞的主要候选者。

Soft X-ray microscopy in the spectral region of "carbon window" was suggested as a way to observe a living cell. A newly-developed cryogenic sample system with the use of liquid nitrogen and oxygen gas was installed in the soft X-ray microscope capable of performing a "carbon window" observation. Using the microscope, alleviation of radiation damage in polystyrene latex spheres at cryogenic temperatures was verified. We have also successfully made an experimental observation of biospecimens at cryogenic temperature. Furthermore, tellurium produced an effect on an image contrast enhancement for Deinococcus radiodurans which is a major candidate for a living cell observation in "carbon window".