Developments of selective detection methods of oligosaccharides bound to receptor molecule

已经开发了各种新的方法来检测蛋白质-配体复合体的相互作用。虽然以往的研究一般采用含100%D_2O的样品溶液，但为了避免样品损失，需要新的方法来处理95%的H_2O和5%的D_2O。在这项研究项目中，还实现了对配体信号的有效和灵敏的检测，在水附近发生共振。这些方法有望在化学和制药领域得到应用。

Various new methods to detect interactions of the protein-ligandcomplex have been developed. Although sample solutions containing 100% D_2O weregenerally used in the previous studies, new methods, which can handle 95% H_2O and 5% D_2O, were required on the point of easy sample preparation with avoiding sample loss. Effective and sensitive detection of ligand signals, resonating close to water, was alsoperformed in this research project. These methods are expected to be useful in thechemical and pharmaceutical fields.