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Study of the interrelationship between Legionella pneumophila and free-living amoeba under the condition of natural water temperature to solving legionaires' disease

自然水温条件下嗜肺军团菌与自由生活阿米巴原虫相互关系研究解决军团病

基本信息

批准号：
21560573
负责人：
OHNO Akira
金额：
$ 3万
依托单位：
Toho University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

It was demonstrated that L. pneumophila shifted into a VNC(Viable but not Culturable) mode inside the Acanthamoeba castellanii cells at a temperature of 15 degrees C by the combination of cultural method, real time PCR(RTPCR) and EMA treated real time PCR(EMA RTPCR), and by transmission electron microscope observation. On the other hand, the shift into VNC state was not observed in Acanthamoeba polyphaga and Haltomannella vermiformis. Culturable L. pneumophila cells were not detected in periodic survey of river water at fixed-sampling points targeting two major rivers in the Tokyo area. A few viable Legionella cells were detected by the combination of RTPCR and EMA RTPCR, however the numbers of viable cells were approximately-same without relation to season. The detection of free-living amoeba(FLA) by PCR showed that the genus Haltomanella was predominant in the sampling points and A. castellanii was not detected. The absence of FLA species that the interrelationship with L. pneumophila is affected by water temperature or few chances to encounter host amoeba by flow of the river might be cause of this result.

结果表明，L.通过结合培养方法、真实的时间PCR（RTPCR）和EMA处理的真实的时间PCR（EMA RTPCR），并通过透射电子显微镜观察，在15 ℃的温度下，嗜肺阿米巴在卡氏阿米巴细胞内转变为VNC（Viable but not Culturable）模式。另一方面，在多食阿米巴和蠕虫状Haltomannella中未观察到向VNC状态的转变。可培养湖在针对东京地区两条主要河流的固定采样点定期调查河水中未检测到嗜肺细胞。RT-PCR和EMA RT-PCR结合检测到少量活军团菌，但活军团菌数量基本相同，与季节无关。PCR检测自由生活阿米巴（弗拉）结果表明，各采样点以Haltomanella属为主，A。未检测到Castellanii。弗拉物种的缺失表明与L.嗜肺原虫受水温影响或河流水流影响很少有机会接触宿主阿米巴可能是造成这一结果的原因。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interrelationship between Legionella pneumophila and free-living amoeba at a low temperature

低温下嗜肺军团菌与自由生活阿米巴原虫之间的相互关系

DOI：
发表时间：
2009
期刊：
影响因子：
0
作者：
Ohno A;Kato N;Yamaguchi K
通讯作者：
Yamaguchi K

レジオネラから覗く生物進化環境微生物の世界

通过军团菌看到生物进化和环境微生物的世界

DOI：
发表时间：
2009
期刊：
日本温泉科学会雑誌
影响因子：
0
作者：
吉田登;長岡耕平;檀智之;石橋啓史;大野章
通讯作者：
大野章

Relationship between Acanthamoeba castellanii and Legionella pneumophila at a temperature below 20 degree C

20℃以下时卡氏棘阿米巴与嗜肺军团菌的关系

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Akira Ohno;Hisashi Mochizuki;Naoyuki Kato;Yoshikazu Ishii;Kazuhiro Tateda;Takashi Suematsu;Yasuhisa Inoue;Keizo Yamaguchi
通讯作者：
Keizo Yamaguchi