Fusion of affinity techniques for protein concentration and novel proteome analysis

点击翻译按钮获取中文摘要

For the detection of minor protein or small amout of protein, which are vey useful for cancer and the other adult deseases, several approach is proposed using the peptide affinity technology for concentration of the minor protein by washing out the major protein. For the evaluation and promotion of these technology, we tried to develop the treatment of high concentration of standard protein and the graphic analysis method of the 2-DE gel image. These technolgy were applied for the scFv production to identify the culture phase and screening of proteins, which attached specifically to the surface of plastics.