Study for the detection of molecular target protein in cancer tissues by Dynamic Spot Method

动态点法检测癌组织中分子靶蛋白的研究

• 批准号: 16560683
• 负责人: KISHIMOTO Michimasa
• 金额: $ 2.05万
• 依托单位: Kyoto Institute of Technology (2005)Osaka University (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16560683/
• 关键词: 2-DE; Proteome analysis; Image analysis; Protein spot

We developed an image analysis system for the detection of specific cancer protein spots in 2-DE gels. The system can analyze the time courses of silver stained gel images during developing processes, and distinguish the specific protein spots of cancer tissues from ones of non-cancer tissues.We also developed an camera system which include the shaking system for performing the developing operation and stop the shaking intermittently for taking picture of the gel images. The system has an advantage of the free hand operation for taking pictures, and we need not transfer the gel from developing instrument to a camera system.The combination of the image analysis and camera system can separate the overlapping spots, because we can get the information of original separated spots from the former images, and we can detect very faint spots because the system provide the later phase images which show the spots of only a few amount of protein relatively clear.These results were presented in 2004 Annual Meeting of the American Electrophoresis Society.

我们开发了一种图像分析系统，用于检测2-DE凝胶中特定的癌症蛋白斑点。该系统可以分析银染色凝胶图像在显影过程中的时间过程，并区分癌组织和非癌组织的特定蛋白质斑点。我们还开发了一套相机系统，该系统包括用于显影操作的震动系统，并间歇地停止震动以拍摄凝胶图像。该系统具有徒手操作拍照的优点，无需将显影仪上的凝胶转移到照相系统上。图像分析与摄像系统相结合，可以分离出重叠的斑点，因为我们可以从之前的图像中获得原始分离斑点的信息，并且系统提供的后期图像可以检测到非常微弱的斑点，这些斑点只显示少量蛋白质的斑点相对清晰。这些结果发表在2004年美国电泳学会年会上。

项目成果

The separation of oil from an oil–water–bacteria mixture using a hydrophobic tubular membrane

• DOI: 10.1016/j.bej.2004.11.012
• 发表时间: 2005-05
• 期刊: Biochemical Engineering Journal
• 影响因子: 3.9
• 作者: M. Konishi;M. Kishimoto;N. Tamesui;T. Omasa;S. Shioya;H. Ohtake

Recognition of culture state using two-dimensional gel electrophoresis with an artificial neural network,

使用二维凝胶电泳和人工神经网络识别培养状态，

• 发表时间: 2006
• 期刊: Proteomics Vol. 6, 3730-3738(2006) 6
• 作者: N. Izawa;M. Kishimoto;M. Konishi;T. Omasa;S. Shioya;H. Ohtake
• 通讯作者: H. Ohtake

Construction and evaluation of drug metabolizing bioartificial liver support system

药物代谢生物人工肝支持系统的构建与评价

• 发表时间: 2005
• 期刊: Biotechnology Progress Vol.21
• 作者: Omasa T.;Kim K;他5名
• 通讯作者: 他5名

Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture

• DOI: 10.1263/jbb.99.259
• 发表时间: 2005-03-01
• 期刊: JOURNAL OF BIOSCIENCE AND BIOENGINEERING
• 影响因子: 2.8
• 作者: Konishi, M;Kishimoto, K;Ohtake, H
• 通讯作者: Ohtake, H