Analysis of regulations of septin modifications in cytokinesis

septin修饰在胞质分裂中的调控分析

• 批准号: 21570003
• 负责人: KIKUCHI Yoshiko
• 金额: $ 3.08万
• 依托单位: Gakushuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570003/
• 关键词: 分子遺伝; 細胞周期; 細胞質分裂; SUMO化; ユビキチン化; セプチン; ユビキチンリガーゼ; SUMOリガーゼ; 酵母; スモ化; リン酸化; アクチンリング; チェックポイントコントロール; チェックポイント

Budding yeast Cdc3 is a component of septin rings which localize at the neck region andis essential for cytokinesis. Cdc3 was transiently sumoylated by Siz1, a SUMO ligase onlyduring M-phase. Cdc3 was ubiquitinated in Rsp5-dependent way, but the PY-motif of Cdc3was not required for the ubiquitination. We examined the phenotypes of various mutantswhen Siz1-septin or SUMO-septin fusion proteins were over-expressed. Results suggestedthat the septin sumoylation induces the depolymerization of septin rings, and that thesumolylated Siz1 was ubiquitinated by Slx8/Slx5 ubiquitin ligase for its degradation.

芽殖酵母Cdc 3是位于颈部区域的Septin环的一个组成部分，是胞质分裂所必需的。Cdc 3仅在M期被SUMO连接酶Siz 1瞬时SUMO化。Cdc 3以Rsp 5依赖的方式被泛素化，但其PY基序不是必需的。当Siz 1-septin或SUMO-septin融合蛋白过表达时，我们检测了各种突变体的表型。结果表明，septin类泛素化可诱导septin环的解聚，类泛素化的Siz 1被Slx 8/Slx 5泛素连接酶泛素化降解。

项目成果

Solution structures and DNA binding properties of the N-terminal SAP domain of SUMO E3 ligase from Saccharomyces cerevisiae and Oryza sativa.

来自酿酒酵母和稻的 SUMO E3 连接酶 N 端 SAP 结构域的溶液结构和 DNA 结合特性。

• 发表时间: 2009
• 期刊: Proteins
• 影响因子: 2.9
• 作者: Suzuki,R.;et al.
• 通讯作者: et al.

Regulations of Mih1-phosphatase in buddingyeast

Mih1-磷酸酶在芽殖酵母中的调控

• 发表时间: 2009
• 作者: 松岡聡;原弘志;松本幸次;Kouitiro Yano and Yoshiko Kikuchi
• 通讯作者: Kouitiro Yano and Yoshiko Kikuchi

Structural analysis of compounds withactions similar to local anesthetics andantipsychotic phenothiazines in yeast

酵母中具有类似局麻药和抗精神病药吩噻嗪类作用的化合物的结构分析

• DOI: 10.1002/yea.1846
• 发表时间: 2011
• 期刊: Yeast
• 影响因子: 2.6
• 作者: Yukifumi Uesono;Akio Toh-e;YoshikoKikuchi;Ichiro Terashima
• 通讯作者: Ichiro Terashima

Regulations of Mih1-phosphatase in budding yeast

Mih1-磷酸酶在芽殖酵母中的调控

• 发表时间: 2009
• 作者: Kikuchi;Y.
• 通讯作者: Y.

Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome

• DOI: 10.1038/emboj.2008.305
• 发表时间: 2009-02-18
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Saeki, Yasushi;Kudo, Tai;Tanaka, Keiji
• 通讯作者: Tanaka, Keiji