Analysis of the genes involved in the maintenance of mini-chromosomes in yeast.
分析参与酵母微型染色体维持的基因。
基本信息
- 批准号:63580208
- 负责人:
- 金额:$ 1.79万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1989
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Yeast plasmids containing the autonomously replicating sequence(ARS) and the centromere (CEN) can be maintained rather stably as mini- chromosomes in yeast cells. We selected the mutants in which mini- chromosomes were unstable at a higher temperature and examined those phenotypes. Our goal of this project is to define how the gene products function for either replication or partitioning.By using the system developed by Hartwell et al., we newly selected three ts mutants; two mutants formed pink colonies with white sectors (presumably defective in replication), one formed pink colonies with white and red sectors (non-disjunction ?). Now we are trying to clone the genes, by complementing activity of the ts phenotype.The GSTl gene which is essential for Gl-to-S transition, affects the stability of mini-chromosomes. The mini-chromosomes were unstable and easily lost at 34゚C. compared at 26゚C. The gene product (MW. 77K) has a homologous domain to polypeptide chain elongation factor lalpha. This gene was found to be the same as the frame-shift suppressor SUFl2, omnipotent suppressor sup2 (sup36) and allosuppressor sal3. We are in progress in studying the function of this protein.We also cloned the human homologue of the yeast GSTl gene from cDNA library of human fibroblast. The gene coded for a protein smaller than yeast GSTl but contained the complete domain homologous to EFlalpha and the consensus sequence for GTP-binding proteins. The mRNA level of the human GSTl gene was the maximum just before entering the S-phase.
含有自主复制序列(ARS)和着丝粒(CEN)的酵母质粒可以作为微型染色体在酵母细胞中相当稳定地维持。我们选择了微型染色体在较高温度下不稳定的突变体并检查了这些表型。我们这个项目的目标是定义基因产物如何发挥复制或分配的功能。通过使用 Hartwell 等人开发的系统,我们新选择了三个 ts 突变体;两种突变体形成具有白色扇区的粉红色菌落(可能是复制缺陷),一种形成具有白色和红色扇区的粉红色菌落(不分离?)。现在我们正试图通过补充ts表型的活性来克隆这些基因。GST1基因是G1到S转变所必需的,它影响微型染色体的稳定性。微型染色体在34℃下不稳定且容易丢失。与 26°C 时相比。基因产物(MW.77K)具有与多肽链延长因子lα同源的结构域。发现该基因与移码抑制子SUF12、全能抑制子sup2 (sup36)和同种异体抑制子sal3相同。我们正在研究该蛋白的功能。我们还从人成纤维细胞的cDNA文库中克隆了酵母GST1基因的人同源物。该基因编码比酵母GST1更小的蛋白质,但含有与EF1α同源的完整结构域和GTP结合蛋白的共有序列。人GST1基因的mRNA水平在进入S期之前达到最高。
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
S. Hoshino et al.: "A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed ina proliferation dependent manner in mammalian cells." EMBO J. 8 3807-3814, 1989.
S. Hoshino 等人:“酵母 GST1 基因的人类同源物编码 GTP 结合蛋白,并在哺乳动物细胞中以增殖依赖性方式表达。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Kikuchi,Y.Kitazawa,H.Shimatake and M.Yamamoto: "The primary structure of the leu1+gene of Schizo-Saccharomyces pombe." Curr.Genetics. 14. 375-379 (1988)
Y.Kikuchi、Y.Kitazawa、H.Shimatake 和 M.Yamamoto:“裂殖酵母 leu1 基因的一级结构。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
A. Fujita et al.: "Domains of the SFL1 protein of yeasts are homologous to Myc onco-proteins or yeast heat-shock transcription factor." Gene 85 321-328, 1989.
A. Fujita 等人:“酵母 SFL1 蛋白的结构域与 Myc 癌蛋白或酵母热休克转录因子同源。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Kikuchi.: "GSTL:A Homolog of polypeptide chain elongation factor is responsible for the stability of mini-chromosomes in Saccharomyces cerevisiae in Geue Expression and Regulation" Elsevier Science Publishers, 257-264 (1988)
Y.Kikuchi.:“GSTL:多肽链延伸因子的同源物负责 Geue 表达和调节中酿酒酵母微型染色体的稳定性”Elsevier Science Publishers,257-264 (1988)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KIKUCHI Yoshiko其他文献
KIKUCHI Yoshiko的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KIKUCHI Yoshiko', 18)}}的其他基金
Analysis of regulations of septin modifications in cytokinesis
septin修饰在胞质分裂中的调控分析
- 批准号:
21570003 - 财政年份:2009
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of the G2/M transition of the budding yeast cell cycle
芽殖酵母细胞周期G2/M转变的机制
- 批准号:
14390015 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and genetical analysis of inducing factors for the G2/M transition in yeast.
酵母 G2/M 转变诱导因素的分子和遗传学分析。
- 批准号:
08680736 - 财政年份:1996
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Expression and localization of the gene products necessary for cell proliferation
细胞增殖所需基因产物的表达和定位
- 批准号:
04680254 - 财政年份:1992
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Studies on the factors affecting gene expression in yeast.
酵母基因表达影响因素的研究。
- 批准号:
61580225 - 财政年份:1986
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
Stable Maintenance of an Extrachromosomal Selfish DNA Element
染色体外自私 DNA 元件的稳定维持
- 批准号:
8126609 - 财政年份:2010
- 资助金额:
$ 1.79万 - 项目类别:
Analysis of centromere proteins for stable maintenance of human artificial chromosome
分析着丝粒蛋白以稳定维持人类人工染色体
- 批准号:
19770153 - 财政年份:2007
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Stable maintenance of an extrachromosomal selfish DNA
染色体外自私DNA的稳定维持
- 批准号:
6415015 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Stable Maintenance of an Extrachromosomal Selfish DNA Element
染色体外自私 DNA 元件的稳定维持
- 批准号:
7674140 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Stable Maintenance of an Extrachromosomal Selfish DNA Element
染色体外自私 DNA 元件的稳定维持
- 批准号:
7191904 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Stable maintenance of an extrachromosomal selfish DNA
染色体外自私DNA的稳定维持
- 批准号:
6736898 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Stable maintenance of an extrachromosomal selfish DNA
染色体外自私DNA的稳定维持
- 批准号:
6887434 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Stable Maintenance of an Extrachromosomal Selfish DNA Element
染色体外自私 DNA 元件的稳定维持
- 批准号:
7365249 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Stable maintenance of an extrachromosomal selfish DNA
染色体外自私DNA的稳定维持
- 批准号:
6620299 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别:
Stable maintenance of an extrachromosomal selfish DNA
染色体外自私DNA的稳定维持
- 批准号:
6618722 - 财政年份:2002
- 资助金额:
$ 1.79万 - 项目类别: