Gonadal plasticity of DMRT1 mutants in the medaka

青鳉DMRT1突变体的性腺可塑性

• 批准号: 21570059
• 负责人: MATSUDA Masaru
• 金额: $ 3.16万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570059/
• 关键词: 形態形成; 生殖腺性分化; メダカ; 性決定; DMRT1遺伝子; 性差形成機構

Dmrt1, which is found in many verte brates, exhibits testis-specific expression during the sexual differentiation period, suggesting a conserved function of Dmrt1 in the testicular development of vertebrate gonads. However, functional analyses have been reported only in mammals. The current study focused on the Dmrt1 function in the teleost medaka, Oryzias latipes, which has an XX-XY sex determina tion system. Although medaka sex is determined by the presence or absence of the Y chromosome-specific gene Dmy, we demonstrated that in one Dmrt1 mutant line, which was found by screening a gene-driven mutagenesis library, XY mutants developed into nor mal females and laid eggs. These results show that Dmrt1 is essential to maintain testis differentiation after Dmy-triggered male differentiation pathway.

Dmrt 1在许多脊椎动物中发现，在性分化期间表现出睾丸特异性表达，表明Dmrt 1在脊椎动物性腺的睾丸发育中具有保守的功能。然而，功能分析仅在哺乳动物中报道。本研究主要探讨了Dmrt 1基因在具有XX-XY性别决定系统的青鳉中的功能。虽然青鳉性别是由Y染色体特异性基因Dmy的存在或不存在决定的，但我们证明了在一个Dmrt 1突变系中，通过筛选基因驱动的诱变文库发现，XY突变体发育成正常雌性并产卵。这些结果表明，Dmrt 1是必不可少的，以维持睾丸分化后，Dmy触发的雄性分化途径。

项目成果

DMRT1遺伝子突然変異メダカはオスからメスへ性転換する

DMRT1基因突变青鳉鱼性别由雄性变为雌性

• 发表时间: 2009
• 作者: Masuyama H;Nagahama Y;Matsuda M;Masaru Matsuda;氣多澄江;増山治男,松田勝
• 通讯作者: 増山治男,松田勝

Sex Determination. in BGM Jamieson (ed.) Reproductive Biology and Phylogeny of Fishes (Agnathans and Bony Fishes)

性别决定。

• 发表时间: 2009
• 作者: Matsuda M;Sakaizumi M
• 通讯作者: Sakaizumi M

Molecular phylogeny and genetic differentiation of Tanakia himantegus complex(Teleostei : Cyprinidae)in Taiwan and mainland China

台湾与中国大陆Tanakia himantegus Complex（Teleostei : Cyprinidae）的分子系统发育和遗传分化

• 发表时间: 2009
• 期刊: Zoological Studies 48
• 作者: Chia-Hao Chang;et al
• 通讯作者: et al

メダカ性決定遺伝子DMYは、いつ性分化の決め手となるのか?

青鳉性别决定基因DMY什么时候成为性别分化的决定因素？

• 发表时间: 2009
• 作者: Konno;N.;Hyodo;S.;Yamaguchi;Y.;Matsuda;K.;Uchiyama;M.;増山治男,松田勝
• 通讯作者: 増山治男,松田勝

Expression of gonadal soma derived factor (GSDF) is spatially and ternporally correlated with early testicular differentiation in medaka

青鳉性腺体衍生因子（GSDF）的表达在空间和时间上与早期睾丸分化相关

• 发表时间: 2010
• 期刊: Gene Expression Patterns
• 影响因子: 1.2
• 作者: Shibata;et al
• 通讯作者: et al