Morphological and molecular based difference between the sibling species of parasitic nematode Anisakis simplex

单异尖线虫（Anisakissimplex）是一种鲸类寄生线虫，通过分子生物学分析，将其分为三个姊妹种，即单异尖线虫（Anisakissimplex）和单异尖线虫（Anisakissimplex）. e. A. simplex sensu stricto，A. pegreffii、黑腹拟步行虫A.单形C. A. simplex sense stricto和A.还报道了在人工培养基中从第三期幼虫培养的成年雄性蠕虫中的佩雷菲氏病。然而，在本研究中，形态分化成年蠕虫收集鲸类胃是失败的，蠕虫的形态是不同的兄弟物种无关。

Anisakis simplex, a cetacean parasitic nematode is differentiated into three sibling species by molecular based analyses, i. e. A. simplex sensu stricto, A. pegreffii, A. simplex C. Morphological differences between A. simplex sensu stricto and A. pegreffii are also reported in adult male worms cultured from third-stage larvae in an artificial medium. However in the present study, morphological differentiation in adult worms collected from the cetacean stomach is failed and the morphology of worms is diverse irrespective of sibling species.