Molecular phylogenetics of diphyllobothriid cestodes with special reference to the species identification of Sparganum proliferum.

二叶绦虫的分子系统发育，特别是三棱的物种鉴定。

• 批准号: 11670256
• 负责人: KURAMOCHI Toshiaki
• 金额: $ 0.58万
• 依托单位: National Science Museum, Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670256/
• 关键词: Sparganum ploriferum; Diphyllobothriidae; Marine Mammals; PCR; COI; NDIII; Molecular Phylogenetics

Sparganum proliferum (Ijima, 1905) is a larval cestode and an etiological agent of proliferative human sparganosis, which is characterized by its agamogenesis by continuous branching and budding in the human body, and invasions on every kind of organs and tissues. Since the first finding by Ijima (J.Coll.Sci.Imp.Univ.Tokyo, 20 : 1-21, 1905), a total of 14 clinical cases has been reported in the world and all of them were fatal. The adult stage of this larval cestode and the life cycle including the final and intermediate hosts in nature has not yet been known in spite of the high medical importance of proliferative human sparganosis.Previous works on S.proliferum has been concentrated in comparison with a putative related species, Spirometra erinaceieuropaei (Rudolphi, 1819)[Platyheiminth : Cestoda : Pseudophyllidea : Diphyllobothriidae], of which larval stage plerocercoid also causes human sparganosis. However significant definitions of S.proliferum are the larval agamous development … More and mortal disease caused by it. Thus, earlier works were summarized that S.proliferum might be abnormally developed Spiro.erinaceieuropaei. While, recently, Kokaze et al.(Parasitol.Internat., 46 : 271-279, 1997) analyzed NADH dehydrognase subunit I (NDIII) gene encoded in the mitochondrial DNA (mtDNA) and revealed that S.proliferum was closely related with Spiro.erinaceieuropaei but both contained differentiable NDIII gene sequence.In this study, we focused on species identification of S.proliferum based on molecular phylogenetic studies. First, we analyzed mitochondrial cytochrome c subunit I (COI) gene sequence of S.proliferum, Spiro.erinaceieuropaei and Diphyllobothrium nihonnkaiense Yamane et al., 1986 [Diphyllobothriidae] to construct phylogenetic tree including teniid cestodes [Cyclophyllidea : Teniidae], and revealed that S.proliferum clearly identified as an pseudophyllidean cestode, also showing high sequence similarity with Spiro.erinaceieuropaei. Second, six species of diphyllobothriids obtained from marine mammalian hosts were added in the analyses. Every phylogenetic analysis failed to find out the species which contained identical sequences or more closely related with S.proliferum, but revealed that the divergence between the cluster included S.proliferum and Spiro.erinaceieuropaei, and the marine species cluster had taken place in earlier period. In addition, the genetic distance between S.proliferum and Spiro. erinaceieuropaei was comparative with or more distant than those between marine species. These results indicate that S.proliferum derived from fresh water origin and may be distinctive species from Spiro.erinaceieuropaei. Less

增殖裂头蚴（Sparganumproliferum，Ijima，1905）是一种绦虫幼虫，是人类增殖性裂头蚴病的病原体，其特点是在人体内不断分枝、出芽，无配子生殖，可侵袭各种器官和组织。自从Ijima首次发现（J.科尔. Sci.Imp. Univ.Tokyo，20：1 - 21，1905）以来，全世界共报道了14例临床病例，并且所有病例都是致命的。尽管增殖性人类裂头蚴病具有很高的医学意义，但这种绦虫幼虫的成虫阶段以及自然界中包括最终宿主和中间宿主的生活史尚未被了解。（Rudolphi，1819）[Platyheiminth：Cestoda：Pseudophyllidea：Diphyllobothiidae]，其中幼虫阶段plerocercoid也引起人类裂头蚴病。然而，增殖链霉菌的重要定义是幼虫的无性生殖发育 ...更多信息 因此，早期的工作总结了S. proliferum可能是异常发育的Spiro.erinaceieuropaei。而最近，Kokaze et al.（Parasitol. Internat.，四十六：271 - 279，1997）分析了线粒体DNA（mtDNA）中编码的NADH脱氢酶亚基I（NDIII）基因，发现S. proliferum与Spiro. erinaceieuropaei关系密切，但都含有可区分的NDIII基因序列。本研究重点是基于分子系统发育研究对S. proliferum进行物种鉴定。首先，我们分析了增殖链霉菌、刺猬螺菌和日本海裂头绦虫的线粒体细胞色素C亚基I（COI）基因序列，1986 [Diphyllobothriidae]构建了包括绦虫科绦虫[Cyclophyllidea：Teniidae]的系统发育树，结果表明，S. proliferum明显鉴定为假叶绦虫，与Spiro.erinaceieuropaei也显示出高度的序列相似性。其次，在分析中增加了从海洋哺乳动物宿主中获得的六种双裂头绦虫。每一次系统发育分析都未能找到与增殖链霉菌序列相同或亲缘关系更近的物种，但发现聚类之间的分歧包括增殖链霉菌和欧洲螺菌，海洋物种聚类发生在更早的时期。此外，增殖链霉菌与螺菌的遗传距离也较远。erinaceieuropaei与海洋种间的亲缘关系较近或较远。这些结果表明，S. proliferum起源于淡水，可能是区别于Spiro.erinaceieuropaei的物种。少

项目成果

Miyadera, H., A.Kokaze, T.Kuramochi, K.Kita, R.Machinami, O.Noya, B.A.de Noya, M.Okamoto and S.Kojima: "Phylogenetic identification of Sparganum proliferum as a pseudophyllidean cestode by the sequence analyses on mitochondrial COI and nuclear sdhB genes.

Miyadera, H., A.Kokaze, T.Kuramochi, K.Kita, R.Machinami, O.Noya, B.A.de Noya, M.Okamoto 和 S.Kojima：“通过序列将三棱豆系统发育鉴定为假叶绦虫

Miyadera,H.,A.Kokaze,T.Kuramochi,K.Kita,R.Machinami,O.Noya,B.A.de Noya,M.Okamoto and S.Kojima: "Phylogenetic identification of Sparganum proliferum as a pseudophyllidean cestode by the sequence analyses on mitochondrial COI and nuclear sdhB genes."Parasit

Miyadera,H.,A.Kokaze,T.Kuramochi,K.Kita,R.Machinami,O.Noya,B.A.de Noya,M.Okamoto 和 S.Kojima：“通过序列将三棱草作为假叶绦虫进行系统发育鉴定

Miyadera et al.: "Phylogenetic identification of Sparganum proliferum as a pseudophyllidean cestode by the sequence analyses on mitochondrial COI and nuclear sdhB genes."Parasitology International. (in press).

Miyadera 等人：“通过对线粒体 COI 和核 sdhB 基因的序列分析，将三棱草鉴定为假叶绦虫。”国际寄生虫学。