Clarification of the physiological role of diacylglycerol in E. coli

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To clarify the physiological role of diacylglycerol in E. coli, ～10 genes, which may be involved in diacylglycerol biosynthesis, were listed up, and then constructed multiply knock-out strains. Resultantly, an eightfold knock-out strain could be constructed, of which the growth was cold-sensitive and the diacylglycerol content was greatly reduced. However, the depletion was not complete. On the other hand, we determined the structure and analyzed the function of glycolipid MPIase involved in protein integration into membranes.