Elucidation of Activation Mechanism of Extracellular Matrix Protein in Related to Allergosis

人骨膜蛋白是一种与变态反应有关的细胞外基质蛋白。我们利用大肠杆菌成功地制备了人骨膜蛋白的功能区（R1-R4），包括四个连续的fasciclin 1样结构域。coli表达系统结合复性方法。连续地，通过凝胶过滤分析选择具有足够的抗原结合能力的两种抗人骨膜蛋白抗体，然后使用R1-R4和来自这些抗体的Fab之间的复合物进行结晶实验。

Human periostin is an extracellular matrix protein in related to allergosis. We succeeded in preparation of functional region(R1-R4), including four sequential fasciclin 1 like domains, of human periostin using E. coli expression system combined with refolding method. Continuously, two anti-human periostin antibodies with sufficient binding ability to antigen were selected by gel filtration analysis, and then crystallization experiments using complex between R1-R4 and Fabs from these antibodies were carried out.