正常意識下の霊長類における視床下部ホルモンの分泌動態の解明

阐明正常意识下灵长类动物下丘脑激素的分泌动态

• 批准号: 01480398
• 负责人: TAMADA Taro
• 金额: $ 3.9万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480398/
• 关键词: hypothalamus; GnRH; GHRH; CRH; hypothalamic hormone; primate; LHーRH

We have developed a new method for monitoring the releasing patterns of the hypothalamic hormones in conscious monkeys. Female castrated monkeys (Macaca fuscata) were used after implamtation of a silasdc capsule containing estradiol. Under anesthesia, the orbital contents were removed. A double lumen needle was inserted along the medial wall of the superior orbital fissure into the upper part of the pituitary adjacent to the stalk. Heparinized saline was constantly fed into the infusion tube at a rate of 1.6ml / 15min. Every 15-min faction for a consecutive 12-h or longer period was analyzed for hypothalamic hormones by a RIA. Blood samples obtained before and during the experiment also were analyzed for GH and cortisol by a RIA.Growth hormone - releasing hormone (GHRH) often increased from base levels to a peak. GHRH pulses in all animals were 60 in 84h. Themean (<plus-minus>SEM) GHRHpulseintervalwas79.5<plus-minus>6.3min. ThemeanpeakievelofGHRHpulseswas<plus-minus>2. lpg/15min, rangi … More ng from7. OtolO5pg/15min. The intervals between major GHRH pulses (peak value>15pg/15min ; increment>lopg/ 15min) were 148.0<plus-minus>16.4min, ranging from 30 to 300min. Seventy - six percent of GH pulses were found to synchronize with the GHRH pulses. Plasma cortisol was remarkably increased during the perfusate collections, suggesting surgical stress. Our findings suggest that the changes of the hypothalamic hormone in the perfusate collected by our procedure represent its secretary patterns from the hypotIn other groups of animals the similar experiments have been performed to elucidate the releasing patterns of corticotropin-releasing hormone (CRID and gonadotropin - releasing hormone (ONRH) in monleys under the same experimental conditions. Female castrated monkeys, were used after sc implantation of a silastic capsule containing estradiol.Although several methods have been developed for assessing the releasing pattems of hypothalamic hormones, such as portal blood collection (Carmel et al., 1976 ; Neill et al., 1977), the push - pull perfusion of the hypothalamus (Levine et al., 1982), some technical and theoretical problems restrict their wide use. Our technique, developed as an alternative method, permits the hypothalamic hormonal release in unanesthesized animals. Less

我们开发了一种新的方法来监测清醒猴子下丘脑激素的释放模式。雌性去势猕猴(Macaca Fuscata)在植入含有雌二醇的硅胶胶囊后被使用。麻醉下摘除眼眶内容物。将双腔穿刺针沿眶上裂内侧壁插入垂体柄附近的垂体部。肝素化生理盐水以1.6ml/15min的速度持续注入输液管。每隔15分钟连续12小时或更长时间，用放射免疫法分析下丘脑激素水平。实验前和实验期间采集的血液样本也用放射免疫法分析了GH和皮质醇。生长激素释放激素(GHRH)通常从基础水平上升到一个峰值。所有动物在84h内GHRH脉搏均为60次。Themean(&lt；+-&gt；sem)GHRHpulseintervalwas79.5&lt；plus-minus&gt；6.3min.ThemeanpeakievelofGHRHpulseswas&lt；plus-minus&gt；2.液化石油气/15分钟，兰吉…从7开始更多的NG。O5pg/15分钟。主要促生长激素释放激素脉冲(峰值15pg/15min；增量10pg/15min)之间的间隔为148.0+lt；正负16.4min，范围30~300min。发现76%的GH脉冲与GHRH脉冲同步。血浆皮质醇在灌流液收集过程中显著升高，表明手术应激。我们的发现表明，本程序采集的灌流液中下丘脑激素的变化代表了其分泌模式。在其他动物组中，也进行了类似的实验，以阐明在相同实验条件下促肾上腺皮质激素释放激素(CRID)和促性腺激素释放激素(ONRH)的释放模式。虽然已发展了几种方法来评估下丘脑激素的释放模式，如门脉采血(Carmel等人，1976；Neill等人，1977)，下丘脑推拉灌注法(Levine等人，1982)，但一些技术和理论问题限制了它们的广泛应用。我们的技术，作为一种替代方法，允许非麻醉动物的下丘脑荷尔蒙释放。较少

项目成果

荒木 重雄: "A prolonged monitoring of the bypothalamic hormonal release in monkeys (Macaca fuscata):Releasing potterns of growth hormoneーreleasing hormone" 内分泌学会誌.

Shigeo Araki：“对猴子（Macaca fuscata）下丘脑激素释放的长期监测：释放生长激素释放激素的罐子”内分泌学会杂志。

荒木重雄: "A prolonged monitoring of the hypothalamic hormanal release in monkeys(Macaca fuscata).Releasing patterns of corticotropin-releasing hormone(CRH)and gonadotropin-releasing hormone(GnRH)" 内分泌学会誌.

Shigeo Araki：“对猴子（Macaca fuscata）下丘脑激素释放的长期监测。促肾上腺皮质激素释放激素（CRH）和促性腺激素释放激素（GnRH）的释放模式”内分泌学会杂志。

Baldwin, D. M and Sawyer, C. H.: "Effects of dexamethasone on LH release and ovulation in the cycling rat." Endocrinology. 94. 1393-1403 (1974)

Baldwin, D. M. 和 Sawyer, C. H.：“地塞米松对循环大鼠 LH 释放和排卵的影响。”

Bullen, B. A., et al: "Induction of men-strual disorders by strenuous exercise in untrained women." N. Engl. J. Med.312. 1349-1353 (1985)

Bullen, B. A. 等人：“未经训练的女性剧烈运动会诱发月经失调。”

Carmel, P. W., et al: "Pituitary stalk portal blood in rhesus monkeys : evidence for pulsatile release of gonadotropin releasing-hormone (GnRH)." Endocrinology. 99. 243-248 (1976)

Carmel, P. W. 等人：“恒河猴的垂体柄门脉血：促性腺激素释放激素 (GnRH) 脉冲式释放的证据。”