Single cell analysis of tail regeneration in Xenopus tadpole

为了分析成纤维细胞生长因子和刺猬信号在尾部再生中的直接作用，从非洲爪蟾蝌蚪中分离脊索细胞。分离的未成熟细胞在培养条件下增殖并终末分化为含液泡的细胞。FGF或hedgehog抑制剂可抑制空泡化。在基因表达筛选之后，鉴定了与脊索成熟相关的基因。

To analyze direct effects of fibroblast growth factor and hedgehog signalings in the tail regeneration notochord cells were isolated from Xenopus tadpole. Isolated immature cells proliferated and terminally differentiated into vacuole-containing cells in a culture condition. The vacuolation was inhibited by FGFs or by hedgehog inhibitor. Genes associated with the notochord maturation were identified after a gene expression screening.