Single cell analysis of tail regeneration in Xenopus tadpole

非洲爪蟾蝌蚪尾部再生的单细胞分析

• 批准号: 21570232
• 负责人: MOCHII Makoto
• 金额: $ 3.08万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570232/
• 关键词: アフリカツメガエル; 尾部再生; 脊索; 表皮; 細胞分化; 分化; 液胞

To analyze direct effects of fibroblast growth factor and hedgehog signalings in the tail regeneration notochord cells were isolated from Xenopus tadpole. Isolated immature cells proliferated and terminally differentiated into vacuole-containing cells in a culture condition. The vacuolation was inhibited by FGFs or by hedgehog inhibitor. Genes associated with the notochord maturation were identified after a gene expression screening.

为了分析成纤维细胞生长因子和刺猬信号在尾部再生中的直接作用，从非洲爪哇蝌蚪中分离出脊索细胞。分离的未成熟细胞在培养条件下增殖并终末分化为含空泡的细胞。FGFs或Hedgehog抑制剂均能抑制空泡化。经过基因表达筛选，确定了与脊索成熟相关的基因。

项目成果

C38, equivalent to BM88, is developmentally expressed in maturing retinal neurons and enhances neuronal maturation

• DOI: 10.1111/j.1471-4159.2009.06536.x
• 发表时间: 2010-03
• 期刊: Journal of Neurochemistry
• 影响因子: 4.7
• 作者: T. Wakabayashi;J. Kosaka;M. Mochii;Y. Miki;Tetsuji Mori;Y. Takamori;H. Yamada

Schwann Cell Precursors from Nerve Innervation Are a Cellular Origin of Melanocytes in Skin

• DOI: 10.1016/j.cell.2009.07.049
• 发表时间: 2009-10-16
• 期刊: CELL
• 影响因子: 64.5
• 作者: Adameyko, Igor;Lallemend, Francois;Ernfors, Patrik
• 通讯作者: Ernfors, Patrik

Xenopus ES1 is expressed in the wound epidermis of regenerating tail and limb bud in tadpole

非洲爪蟾ES1在蝌蚪再生尾部和肢芽的伤口表皮中表达

• 发表时间: 2012
• 作者: Shigehiro Yasukawa;Kaname Yoneda;Akira Tazaki;Kenji Watanabe;Makoto Mochii
• 通讯作者: Makoto Mochii

In vitro studies on migratory activity of Xenopus primordial germ cells

非洲爪蟾原始生殖细胞迁移活性的体外研究

• 发表时间: 2011
• 作者: Terayama K;Kataoka K;Morichika K;Orii H;Watanabe K;Mochii M
• 通讯作者: Mochii M

Molecular mechanism of primordial germ cell migration in Xenopus

非洲爪蟾原始生殖细胞迁移的分子机制

• 发表时间: 2009
• 作者: Kohei Terayama;Kensuke Kataoka;Keisuke Morichika;Kenji Watanabe;Hidefumi Orii;Makoto Mochii
• 通讯作者: Makoto Mochii