Production of Colchicine. derived Tetraploid Wild Vaccinium spp. and Its Use in Breeding

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We collected and evaluated Vaccinium wild species native to Japan, have not been utilize for horticulture up to the present, as genetic resources to breed some blueberry cultivars adaptable to Japan with high functionality. And we tried to obtain some interspecific hybrids among wild species and between blueberry cultivars using reciprocal crossing. We also carried out colchicine treatment of shoots in vitro in some wild species, to produce chromosome-doubled plants from diploid wild species, will easily cross with tetraploid cultivated species.