Construction of recombinant baculoviruses without using cell cultures

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Construction of recombinant viruses is important for elucidating viral gene function and infection processes, as well as for recombinant protein production. However, with the exception of a few model systems, most of the～600 known baculoviruses lack permissive cell lines in which recombinant genotypes can be produced and, consequently, the molecular pathology of those viruses is poorly understood. The purpose of this study was to establish a generally applicable methodology to construct recombinant baculoviruses without having to use cell culture. In this study, the bacmid system was constructed using baculoviruses genome introduced with bacterial replication origin and generated recombinant virus DNA was established.