Functional characterization of two-component high-affinity nitrate transport system

在大麦中，低水平的外部硝酸盐被高亲和力转运系统（HATS）吸收，该系统由两种蛋白质HvNRT 2和HvNAR 2组成。在本研究中，这两种蛋白质在离体质膜中形成复合物，并共定位于根皮层细胞的最外层。此外，双分子荧光互补分析表明，HvNRT 2和HvNAR 2在天然植物细胞的质膜上相互作用。

In barley, low level of external nitrate is absorbed by high affinity transport system(HATS) which is composed of two proteins, HvNRT2 and HvNAR2. In this study, it was suggested that two proteins make complex in the isolated plasma membrane and co-localized at out most layer of the cells in root cortex. In addition, Bimolecular fluorescence complementation analysis showed that HvNRT2 and HvNAR2 interacts in the plasma membrane of the native plant cells.