Analysis of sterol metabolic pathway in hexaploid wheat

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Wheat(Triticum aestivum) is one of the three major crops. It is expected to add value to the crop by the modification of sterol profiles. However, because of the complicated genome(hexaploid) and difficulty of transgenic work, there have been no information of sterol pathway in the crop. In this study, 13 genes for putative sterol metabolic pathway was identified. Among these genes, we elucidated the CYP710A8 encoding sterol C-22 desaturase as a key characteristic for the higher level of stigmasterol. The gene was mapped on chromosome 3(3A, 3B, and 3D) and difference of the function of the genes was confirmed by overexpression of each gene in Arabidopsis.