Studies on the structure and function of enzymes related to C-S bond formation and cleavage of useful naturally-occuring cyclic compounds having sulfur

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The structure and function of thermophilic enzymes related to C-S bond formation and cleavage of naturally occuring important cyclic compounds were studied for the first time. Targeted enzymes are KAPA synthase(BioF), which is involved in the first step of biotin(vitamin H) synthetic pathway, as the model enzyme related to C-S bond formation pathway, and dibenzothiophene(DBT) monooxigenase(BdsC), which is involoved in the first and second steps of DBT, an air pollutant contained in petroleum, as the model enzyme related to C-S bond cleavage pathway. As for the former enzyme, the temperature properties and substrate specificities were significantly superior to those of known mesophilic organisms. As for the latter enzyme, a useful new reaction was found in terms of application.