Studies on the structure and function of enzymes related to C-S bond formation and cleavage of useful naturally-occuring cyclic compounds having sulfur

与有用的天然存在的含硫环状化合物的C-S键形成和裂解相关的酶的结构和功能的研究

• 批准号: 21580093
• 负责人: IZUMI Yoshikazu
• 金额: $ 3.16万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580093/
• 关键词: 好熱性微生物; 好熱性酵素; 環状含硫有機化合物; ビオチン生合成系酵素; ジベンゾチオフェン脱硫酵素系; 炭素-硫黄結合; Bio酵素; バイオインフォマティクス; bio遺伝子; BioF; DBT脱硫代謝系の酵素; Bio遺伝子; Dsz遺伝子; 酵素ライブラリー; 環状含硫化合物

The structure and function of thermophilic enzymes related to C-S bond formation and cleavage of naturally occuring important cyclic compounds were studied for the first time. Targeted enzymes are KAPA synthase(BioF), which is involved in the first step of biotin(vitamin H) synthetic pathway, as the model enzyme related to C-S bond formation pathway, and dibenzothiophene(DBT) monooxigenase(BdsC), which is involoved in the first and second steps of DBT, an air pollutant contained in petroleum, as the model enzyme related to C-S bond cleavage pathway. As for the former enzyme, the temperature properties and substrate specificities were significantly superior to those of known mesophilic organisms. As for the latter enzyme, a useful new reaction was found in terms of application.

首次研究了与C-S键形成和重要环状化合物裂解有关的嗜热酶的结构和功能。目标酶是生物素（维生素H）合成途径第一步的KAPA合成酶（BioF），作为C-S键形成途径的模型酶;二苯并噻吩（DBT）单加氧酶（BdsC），作为C-S键断裂途径的模型酶，参与石油中的空气污染物DBT的第一步和第二步。对于前一种酶，其温度特性和底物特异性明显上级已知的嗜温生物。对于后者，在应用方面发现了一个有用的新反应。

项目成果

Detection and Characterization of a Thermophilic Biotin Biosynthetic Enzyme, 7-Keto-8-aminopelargonic Acid Synthase, from Various Thermophiles

来自各种嗜热菌的嗜热生物素生物合成酶 7-Keto-8-氨基壬酸合酶的检测和表征

• 发表时间: 2012
• 期刊: Biosci. Biotechnol. Biochem.
• 作者: M.Goto;K.Seto;T.Futagami;Y.Kajiwara;H.Takashita;T.Omori;K.Takegawa.;Takaaki Kubota and Yoshikazu Izumi
• 通讯作者: Takaaki Kubota and Yoshikazu Izumi

Aminolytic reaction catalyzed by D-stereospecific amidohydrolases from Streptomyces spp

链霉菌属 D-立体特异性酰胺水解酶催化的氨解反应

• 发表时间: 2011
• 期刊: Biochimie
• 影响因子: 3.9
• 作者: 田坂友一、藤江誠、山田隆;他1名;J.Arima
• 通讯作者: J.Arima

Isolation and Characterization of 3-N-Trimethylamino-1-propanol Degrading Rhodococcus sp. strain A2

3-N-三甲氨基-1-丙醇降解红球菌的分离和表征。

• 发表时间: 2009
• 期刊: FEMS Microbiol. Lett.
• 作者: Isam Ali Mohamed Ahmed;Jiro Arima;Tsuyoshi Ichiyanagi;Emi Sakuno and Nobuhiro Mori
• 通讯作者: Emi Sakuno and Nobuhiro Mori

Novel Reactivity of dibenzothiophene mono-oxygenase from Bacillus subtilis WU-S2B

枯草芽孢杆菌 WU-S2B 中二苯并噻吩单加氧酶的新反应性

• 发表时间: 2009
• 期刊: Biosci. Biotechnol. Biochem. 73
• 作者: Takashi Ohshiro;Shuhei Nakura;Yoshitaka Ishii;Kuniki Kino;Kohtaro Kirimura;Yoshikazu Izumi
• 通讯作者: Yoshikazu Izumi

Novel Reactivity of Dibenzothiophene Monooxigenase from Bacillus subtilis WU-S2B

枯草芽孢杆菌 WU-S2B 中二苯并噻吩单加氧酶的新反应性

• 发表时间: 2009
• 期刊: Biosci. Biotechnol. Biochem.
• 作者: Takashi Ohshiro;Shuhei Nakura;Yoshitaka Ishii;Kuniki Kino;Kohtaro Kirimura;and Yoshikazu Izumi
• 通讯作者: and Yoshikazu Izumi