Studies on Distribution of Marine Macro-algae in Europe Which Produce Novel Useful Enzymes and Their Structure-Function
Studies on Distribution of Marine Macro-algae in Europe Which Produce Novel Useful Enzymes and Their Structure-Function
批准号:
15404025
负责人:
IZUMI Yoshikazu
金额:
$7.87万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2005
中文摘要
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英文摘要
Little has been done about biochemical studies on marine algae as an undeveloped genetic resource mainly because of technical difficulty. The purpose of this study is to find novel enzymes from marine macro-algae collected in Japan and Europe. Moreover we aimed to elucidate the structure and function of a unique vanadium-dependent haloperoxidase (VHPO) from a marine alga Corallina sp.During the period of this study, we collected marine algae around Tottori, Exeter, U.K., and Amsterdam, the Netherlands, and then determined various enzyme activities of cell-free extracts of the algae. We found an alga with a high phosphatase activity. The enzyme activity showed an optimal pH of about 8, optimal temperature of 60-70℃, and the 60% and 80% stabilities after the incubation at 60℃ for 30 min and at pH 3.5-9.0, respectively. Furthermore, the enzyme showed the substrate specificity toward fructose 1,6-bisphosphate (53%), glucose 6-phosphate (19%), and glyceraldehyde 3-phosphate (16%) as well as p-nitrophenylphosphate (100%).We examined the factors for thermostability and organic solvent tolerance of VHPO of C.pilulifera. As a result, holo-VHPO showed tolerance against tetrahydrofuran and acetonitrile as well as ethanol and acetone, whereas apo-VHPO showed tolerance only against acetone.From the X-ray analyses of VHPO, we found the calcium atoms in the VHPO molecule. The Ca atom and vanadate, the prosthetic group of the enzyme, were found to play an important part of the enzyme stabilities.
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T.Kawai, T.Kubota, J.Hiraki, Y.Izumi: "Biosynthesis of ε-poly-L-lysine in a cell-free system of Streptomyces albulus"Biochem.Biophys.Res.Commun.. 311. 635-640 (2003)
T.Kawai、T.Kubota、J.Hiraki、Y.Izumi:“白色链霉菌无细胞系统中 ε-聚-L-赖氨酸的生物合成”Biochem.Biophys.Res.Commun. 311. 635-640 (2003)
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大城 隆, 和泉好計: "石油の硫黄・窒素・金属の除去"農芸化学の事典. 757-760 (2003)
Takashi Oshiro、Yoshikei Izumi:“从石油中去除硫、氮和金属”农业化学百科全书 757-760 (2003)。
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[]
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DOI:
10.1110/ps.03496004
发表时间:
2004-06-01
期刊:
PROTEIN SCIENCE
影响因子:
8
作者:
[Ohshiro, T, Littlechild, J, Izumi, Y]
通讯作者:
Izumi, Y
Enhancing effect of calcium and vanadium ions on thermal stability of bromoperoxidase from Corallina pilulifera
钙、钒离子对珊瑚虫溴过氧化物酶热稳定性的增强作用
DOI:
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发表时间:
2005
期刊:
J. Biol. Inorg. Chem. 10
影响因子:
--
作者:
[E.Garcia-Rodriguez, T.Ohshiro, T.Aibara, Y.Izumi, J.Littlechild]
通讯作者:
J.Littlechild
Dibenzothiophene Desulfurizing Enzymes from a Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B - Purification, Characterization and Overexpression-
来自中度嗜热细菌枯草芽孢杆菌 WU-S2B 的二苯并噻吩脱硫酶 - 纯化、表征和过表达 -
DOI:
--
发表时间:
2005
期刊:
J.Biosci.Bioeng. 100(2)
影响因子:
--
作者:
[T.Ohshiro, Y.Ishii, K.Ueda, Y.Izumi, K.Kino, K.Kirimura]
通讯作者:
K.Kirimura
共 8 条
Studies on the structure and function of enzymes related to C-S bond formation and cleavage of useful naturally-occuring cyclic compounds having sulfur
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批准号:21580093
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.16万
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财政年份:2009
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负责人:IZUMI Yoshikazu
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依托单位:
Improvement of Functions of Novel Enzymes in the Desulfurization Metaoblism of Petroleum by Protein Engineering and Molecular Genetics
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批准号:15580063
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.43万
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财政年份:2003
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负责人:IZUMI Yoshikazu
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依托单位:
Elucidation of properties of novel enzymes catalyzing the formation and the cleavage of carbon-sulfur bond in microooganisms
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批准号:11660091
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.98万
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财政年份:1999
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负责人:IZUMI Yoshikazu
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依托单位:
Elucidation of Structure and Reaction Mechanism of Novel Crystalline Enzymes Specific for C1 Microoranisms
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批准号:09044224
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项目类别:Grant-in-Aid for Scientific Research (A).
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资助金额:$9.02万
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财政年份:1997
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负责人:IZUMI Yoshikazu
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依托单位:
Elucidation of functions of novel enzymes catalyzing the formation and the cleavage of cabond and their application
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批准号:09650877
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.05万
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财政年份:1997
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负责人:IZUMI Yoshikazu
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依托单位:
Development of production process for physiologically active substanses using haenzymes from marine alga and microoganism
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批准号:07556092
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$3.01万
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财政年份:1995
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负责人:IZUMI Yoshikazu
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依托单位:
Enzymatic and Molecular Genetic Studies of Serine Production by C_1-Microorganisms
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批准号:06650917
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.28万
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财政年份:1994
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负责人:IZUMI Yoshikazu
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依托单位:
Studies on the Metabolic Enzymes Characteristic to C_1-Microorganisms----Structures and Functions
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批准号:06044148
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$3.14万
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财政年份:1994
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负责人:IZUMI Yoshikazu
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依托单位:
Halogenating Enzymes Produced by Marine Algae and Microbes---Structures and Functions
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批准号:04044118
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$8.13万
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财政年份:1992
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负责人:IZUMI Yoshikazu
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依托单位:
Enzymzyic znd Molecular Genetic Studies of Serine Production by C_1-Microorganisms
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批准号:04660119
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1992
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负责人:IZUMI Yoshikazu
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依托单位:
Studies on the Characterization of Metabolic Properties of C_1-microorganisms and Its Application to Serine Synthesis
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批准号:01560121
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1989
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负责人:IZUMI Yoshikazu
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依托单位:
海外基金