Survival mechanisms of Campylobacter jejuniin co-culture with Acanthoamoebaspp.

空肠弯曲杆菌与棘阿米巴菌共培养的生存机制。

• 批准号: 21580381
• 负责人: KAKUDA Tsutomu
• 金额: $ 3.16万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580381/
• 关键词: カンピロバクター; アメーバ; 伝播; 細胞内生存

Campylobacter jejuniis a leading cause of food poisoning all over the world. Contaminated chicken meats are major source of infection in human. Therefore, reduction of C. jejuni-burden in chickens is an important factor for reduction of risk of infection. However, infection route of C. jejuniamong chickens has not been fully understood. Recently, it is suggested that amoeba could be reservoir of C. jejuni. In this study, we tried to identify the genes involving in growth inside of amoeba by using transposon mutagenesis. As results, it was suggested that internalization of C. jejuniinto amoeba depended on flagellar motility but was not necessary for proliferation of bacteria in co-culture with ameba.

空肠弯曲菌是世界范围内引起食物中毒的主要原因。受污染的鸡肉是人类感染的主要来源。因此，C.鸡的空肠负担是降低感染风险的重要因素。然而，C.鸡群中的jejuniamong鸡还没有被完全理解。近年来，有研究认为阿米巴可能是C.空肠。在本研究中，我们试图通过转座子突变来鉴定变形虫体内与生长有关的基因。结果表明，C. jejunininto阿米巴依赖于鞭毛运动，但不是与阿米巴共培养的细菌增殖所必需的。

项目成果

False Positive Responses of Campylobacter jejuni when Using the Chemical-In-Plug Chemotaxis Assay

• DOI: 10.1292/jvms.10-0396
• 发表时间: 2011-03-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Kanungpean, Doungjit;Kakuda, Tsutomu;Takai, Shinji
• 通讯作者: Takai, Shinji

Characterization of two putative mechanosensitive channel proteins of Campylobacter jejuni involved in protection against osmotic downshock

• DOI: 10.1016/j.vetmic.2012.04.044
• 发表时间: 2012-11-09
• 期刊: VETERINARY MICROBIOLOGY
• 影响因子: 3.3
• 作者: Kakuda, Tsutomu;Koide, Yuuki;Takai, Shinji
• 通讯作者: Takai, Shinji

Participation of CheR and CheB in Chemosensory Response of Campylobacter jejuni

CheR和CheB参与空肠弯曲菌化学感应反应

• 发表时间: 2011
• 期刊: Microbiology
• 影响因子: 1.5
• 作者: Doungjit Kanungpean;Tsutomu Kakuda;Shinji Takai
• 通讯作者: Shinji Takai

Participation of CheR and CheB in chemosensory adaptation of Campylobacter jejuni

CheR和CheB参与空肠弯曲杆菌的化学感应适应

• 发表时间: 2010
• 作者: Doungjit Kanungpean;et al.
• 通讯作者: et al.

Characterization of CheB and CheR in Campylobacter jejuni using site-directed mutagenesis

使用定点诱变表征空肠弯曲杆菌中的 CheB 和 CheR

• 发表时间: 2010
• 作者: Doungjit Kanungpean;角田勤、高井伸二
• 通讯作者: 角田勤、高井伸二