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Study on establishment of the dog frozen semen technique for the purpose of the frozen semen bank establishment of assistance dog

以辅助犬冷冻精液库建立为目的的犬冷冻精液技术建立研究

基本信息

批准号：
21580396
负责人：
HORI Tatsuya
金额：
$ 2.75万
依托单位：
Nippon Veterinary and Life Science University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

It is the present situations in Japan that the assistance dog including the guide dog is very insufficient. Because the lineage is necessary for reproduction of these dogs, it will be necessary to introduce the semen of an excellent dog and breed systematically at earth level in future. The establishment of the canine frozen semen technique that practical use is possible is indispensable. Therefore, for the purpose of establishment of canine frozen semen bank, the development of frozen semen technique providing high conception rate by the vaginal insemination and the establishment of the nonsurgical artificial insemination technique to reduce the aggression on dog were studied.As a result, Egg-yolk tris fructose citric acid extender was effective, and a high conception rate was provided by the surgical intrauterine insemination of canine frozen semen. In addition, it became clear that intrauterine insemination by the semen frozen after preservation at low temperature until 48 hours provided the high conception rate. It was thought that the semen frozen after transport of semen at low temperature from the distant place in Japan or the foreign countries might be useful. However, when we observed the vagina and the external os of uterus before and after the ovulation using the endoscope(fiberscope) for small animals, the insertion of the catheter was possible, but the passage of a catheter was able to insert in only one of 11 bitches. Therefore, I could not examine the conception by the transvaginal intrauterine insemination of canine frozen semen. It in future, it was thought that the expansion of the observable range of the external os of uterus, the development of an air pressure and the development of catheter that enabled it would be necessary.

日本的现状是，包括导盲犬在内的辅助犬非常不足。因为血统是这些狗繁殖所必需的，所以将来有必要引进优秀狗的精液，并在地球水平上系统地繁殖。建立实用化的犬冷冻精液技术是必不可少的。为此，本研究以建立犬冷冻精液库为目的，对提高阴道授精受胎率的冷冻精液技术的开发和降低犬攻击性的非手术人工授精技术的建立进行了研究，结果表明，蛋黄三果糖柠檬酸稀释液效果良好，通过犬冷冻精液的外科子宫内授精提供了高受胎率。此外，很明显，低温保存48小时后冷冻的精液进行宫腔内授精可提供高受胎率。有人认为，从日本或外国遥远的地方低温运输精液后冷冻的精液可能有用。然而，当我们使用小动物内窥镜（纤维镜）观察排卵前后的阴道和子宫外口时，可以插入导管，但导管通道只能插入11只母犬中的1只。因此，我无法通过经阴道宫腔内输注犬冷冻精液来检查受孕情况。它在未来，它被认为是子宫外口的可观察范围的扩大，空气压力的发展和导管的发展，使它成为必要的。