Analysis on the function and coalition of RecQL4, a product of a gene mutated in a progeria syndrome, in the DNA transactions.

早衰症基因突变产物RecQL4在DNA交易中的功能和联合分析。

• 批准号: 21590059
• 负责人: TADA Shusuke
• 金额: $ 3万
• 依托单位: Teikyo Heisei University (2010-2011)Tohoku University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590059/
• 关键词: Rothmund-Thomson症候群; RecQL4; DNA修復; DNA複製; 遺伝子構造安定性維持; 遺伝情報安定性維持機構; 質量分析; ユビキチン化; ニワトリDT40細胞

In this study, we aimed to understand the function and behavior of RecQL4, a product of a gene mutated in a progeria syndrome, in higher eukaryotic cells. Cell-biological or biochemical analyses of RecQL4 revealed that 1) RecQL4 is promptly translocated onto a site of DNA lesions, 2) RecQL4 interacts with numbers of post-translational modification enzymes, as well as proteins relevant to recognition and repair of DNA lesions, and 3) RecQL4 possesses domains for viability of higher eukaryotic cellsand for coping with DNA damagesin separate regions.

在这项研究中，我们的目的是了解RecQL 4的功能和行为，RecQL 4是一种在早衰综合征中突变的基因的产物，在高等真核细胞中。RecQL 4的细胞生物学或生化分析表明：1）RecQL 4能迅速转移到DNA损伤位点上; 2）RecQL 4与许多翻译后修饰酶以及与DNA损伤识别和修复相关的蛋白质相互作用; 3）RecQL 4在不同的区域具有维持高等真核细胞活力和应对DNA损伤的功能域。

项目成果

SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA

• DOI: 10.4061/2010/795946
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF NUCLEIC ACIDS
• 影响因子: 2.3
• 作者: Inoue, Eri;Tano, Keizo;Enomoto, Takemi
• 通讯作者: Enomoto, Takemi

A mutant protein of histone chaperone CIA/Asf1 defective in disrupting histone(H3-H4)_2 tetramer accelerates rate of DNA replication

组蛋白伴侣CIA/Asf1突变蛋白破坏组蛋白(H3-H4)_2四聚体，加速DNA复制速率

• 发表时间: 2009
• 作者: Sasaki M;Hamada K;Kawahara K;Sasaki T;Maehama T;Suzuki A;大隅達也
• 通讯作者: 大隅達也

DNA複製に必須なCdc45の新規機能の解析

DNA复制必需的Cdc45新功能分析

• 发表时间: 2009
• 作者: Horie Y;Kataoka E;Ohshima S;Sato W;Dohmen T;Goto T;Takeuchi S;Iizuka M;Suzuki A;Watanabe S;大隅達也
• 通讯作者: 大隅達也

The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells

缺少解旋酶结构域的 RECQL4 N 末端区域对于脊椎动物细胞的生存能力来说是必需的且足够的

• 发表时间: 2011
• 期刊: Biochim. Biophys. Acta
• 作者: Abe;T.;Yoshimura;A.;Hosono;Y.;Tada;S.;Seki;M.;Enomoto. T.
• 通讯作者: Enomoto. T.

The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells Role of the N-terminal region of RECQL4 in cells

• DOI: 10.1016/j.bbamcr.2011.01.001
• 发表时间: 2011-03-01
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
• 影响因子: 5.1
• 作者: Abe, Takuya;Yoshimura, Akari;Enomoto, Takemi
• 通讯作者: Enomoto, Takemi