The Role of RECQL4 in Bone Development and Osteosarcoma

RECQL4 在骨发育和骨肉瘤中的作用

• 批准号: 7862002
• 负责人: Lisa L Wang
• 金额: $ 34.43万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7862002
• 关键词: Adolescent; Biochemical; Bone Development; Bone Diseases; Bone neoplasms; Calcium; Cell Line; Cells; Child; Clinical Research; Constitutional; Data; Defect; Deposition; Development; Dual-Energy X-Ray Absorptiometry; Event; Gene Proteins; General Population; Genetic; Goals; Hereditary Disease; Homeostasis; Human; Human Genetics; In Vitro; Inherited; Knock-in Mouse; Knockout Mice; Label; Laboratories; Malignant Bone Neoplasm; Malignant Neoplasms; Molecular; Mus; Mutant Strains Mice; Mutate; Mutation; Nature; Organism; Osteoblasts; Osteogenesis; Osteopenia; Osteoporosis; Pathogenesis; Pathway interactions; Patients; Phenotype; Play; Primary Bone Osteosarcoma; Proteins; RECQL4 gene; Radial; Risk; Role; Rothmund-Thomson syndrome; Scanning; Signal Pathway; Signal Transduction; Small Interfering RNA; Study models; Syndrome; Testing; Tissues; Transgenic Mice; Two-Hybrid System Techniques; Work; Yeasts; base; bone; bone cell; bone turnover; cell type; chemotherapy; disease-causing mutation; helicase; high risk; improved; in vitro Assay; insight; member; mouse model; novel; osteogenic; osteosarcoma; overexpression; promoter; protein expression; public health relevance; research clinical testing; skeletal; skeletal abnormality; skeletal dysplasia; substantia spongiosa; tomography; tumorigenesis; tumorigenic

DESCRIPTION (provided by applicant): Rothmund-Thomson syndrome (RTS) is a mutisystem genetic disorder caused by mutations in the RECQL4 gene. Our previous studies characterizing this syndrome have led to several observations regarding bone defects in these patients. RTS patients have severe skeletal abnormalities, including osteopenia, hypoplastic or fused bones, and trabecular defects. They also have a significantly increased risk for developing osteosarcoma (OS), a primary malignant bone tumor for which new therapies are greatly needed. These bone conditions are increased when RECQL4 is mutated, suggesting that RECQL4 plays an important function in normal bone development and in suppression of OS. However, the exact function the RECQL4 DNA helicase and how it exerts its effects in normal bone cells and in bone tumors are not known. We have shown through yeast two-hybrid assay that RECQL4 interacts with PRICKLE1 protein, a member of the Wnt signaling pathway known to be important in both bone development and cancer. The objective of this proposal is to examine the skeletal consequences of loss of RECQL4 function both clinically and at the molecular and cellular level in order to define the mechanism of RECQL4 action in bone. To test our hypothesis that RECQL4 is a bone homeostasis protein that exerts its developmental and tumorigenic effects through the Wnt signaling pathway, we propose the following aims. In Aim 1, we will characterize the human skeletal phenotype that results from loss of RECQL4 function in RTS patients through detailed clinical evaluation, including DXA scanning, biochemical bone turnover markers, and IV calcium labeling studies to assess bone formation and resorption. In Aim 2, we will generate an osteoblast-specific Recql4 conditional knockout (CKO) mouse model to characterize the skeletal defects and cancer phenotypes in Recql4 deficient mice through detailed phenotypic and bone histomorphometric analyses. In Aim 3, we will explore the effects of loss of RECQL4 function on Wnt signaling using in vitro assays of b-catenin activity and gene and protein expression analyses of the Wnt and related signaling pathways. Understanding the skeletal defects in RTS patients and the cellular events in terms of bone formation and resorption will allow us to define the molecular pathways in which RECQL4 participates and how it relates to bone development and tumorigenesis. Results of these studies will guide us in the treatment of RTS patients, provide insight into the mechanisms of bone disease, and may eventually provide opportunities for more targeted therapies for the treatment of OS and osteoporosis in the general population. PUBLIC HEALTH RELEVANCE: The goal of our project is to study the role of the RECQL4 gene pathway in the development of osteosarcoma and other bone diseases such as osteoporosis. We will study patients with a human genetic disorder (Rothmund-Thomson syndrome) along with mouse models of RECQL4 deficiency to understand the cellular and molecular basis of skeletal dysplasia. This will allow us to discover novel ways to improve the treatment of osteosarcoma and osteoporosis in the general population.

描述(申请人提供)：Rothmund-Thomson综合征(RTS)是一种由RECQL4基因突变引起的多系统遗传性疾病。我们之前对这种综合征的研究已经导致了一些关于这些患者的骨缺陷的观察。RTS患者有严重的骨骼异常，包括骨量减少、骨发育不良或融合，以及骨小梁缺陷。他们患骨肉瘤(OS)的风险也显著增加，OS是一种非常需要新疗法的原发恶性骨肿瘤。当RECQL4突变时，这些骨骼条件会增加，这表明RECQL4在正常骨发育和抑制OS中发挥着重要作用。然而，RECQL4 DNA解旋酶的确切功能以及它如何在正常骨细胞和骨肿瘤中发挥作用尚不清楚。我们已经通过酵母双杂交实验证明RECQL4与PRICKLE1蛋白相互作用，PRICKLE1蛋白是Wnt信号通路的成员，已知在骨骼发育和癌症中都是重要的。本研究的目的是从临床、分子和细胞水平研究RECQL4功能缺失对骨骼的影响，以明确RECQL4在骨骼中的作用机制。为了验证我们的假设，即RECQL4是一种骨骼稳态蛋白，它通过Wnt信号通路发挥其发育和致瘤作用，我们提出了以下目标。在目标1中，我们将通过详细的临床评估，包括DXA扫描、生化骨转换标记物和IV钙标记研究来评估骨形成和骨吸收，来表征RTS患者由于RECQL4功能丧失而导致的人类骨骼表型。在目标2中，我们将建立成骨细胞特异性的Recq14条件性基因敲除(CKO)小鼠模型，通过详细的表型和骨组织形态计量学分析来表征RecqL4缺陷小鼠的骨骼缺陷和癌症表型。在目标3中，我们将通过体外b-catenin活性分析和Wnt及其相关信号通路的基因和蛋白表达分析，探讨RECQL4功能丧失对Wnt信号转导的影响。了解RTS患者的骨缺陷以及骨形成和吸收方面的细胞事件将使我们能够定义RECQL4参与的分子途径以及它与骨发育和肿瘤发生的关系。这些研究的结果将指导我们治疗RTS患者，深入了解骨骼疾病的机制，并最终可能为治疗OS和普通人群中的骨质疏松症提供更有针对性的治疗机会。 公共卫生相关性：我们项目的目标是研究RECQL4基因通路在骨肉瘤和其他骨病(如骨质疏松症)发生中的作用。我们将研究患有人类遗传性疾病(Rothmund-Thomson综合征)的患者以及RECQL4缺乏的小鼠模型，以了解骨骼发育不良的细胞和分子基础。这将使我们能够发现新的方法来改善普通人群中骨肉瘤和骨质疏松症的治疗。