Roles of transcription factors during endothelial-mesenchymal transformation in the developing chick heart

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I examined role of sox9 in the endothelial-mesenchymal transformation(EMT) during cushion tissue formation using chick embryonic hearts. First, we examined their expression patterns in the developing heart by in situ hybridization. sox9 was expressed in the activated-endothelial and mesenchymal cells of the outflow tract and the atrioventricular canal regions. Second, sox9 siRNA inhibited endothelial transformation in vitro assay. Endothelial cells transfected with sox9 showed cell migration, but did not invade into the gel lattice. However endothelial cells transfected with sox9 and slug transformed to mesenchymal cells. Taken together, results suggest that sox9 and slug may play an important role during endocardial epithelial-mesenchymal transition