The effects to damaged joints by using hyaluronic acid with various molecular weight

我们检测了透明质酸（HA）酶（HAS）和透明质酸酶（Hyals）在受损关节组织中的表达，以及它们之间的关系。我们还研究了类风湿关节炎（RA）患者滑液中低分子量（MW）HA的细胞毒性。在RA的活动性炎症阶段，滑膜组织中的炎性细胞产生HAS-3，这些细胞可以合成低分子量的HA。在RA活动期，透明质酸酶（Hyal）-1和Hyal-2阳性细胞增多，HA可能发生变性，分子量降低。在某些RA病例中，滑液中Hyal活性较高。低分子量的HA可能导致滑液作为润滑剂的功能障碍，并且它也具有促炎作用，因此HAS 1、2、3或Hyals之间的平衡对于维持关节的正常功能可能是重要的。

We examined the expression of hyaluronic acid(HA) synthases(HASs) and Hyaluronidases(Hyals) in damaged articular tissues, and the relationship between them. We also examined the cytotoxicity of HA with low molecular weight(MW) in the synovial fluid of patients with rheumatoid arthritis(RA). In active inflammatory stage of RA, inflammatory cells in synovial tissue produce HAS-3 and the cells may synthesize HA with low MW. In active inflammatory stage of RA, the number of positive cells of hyaluronidase(Hyal)-1 and Hyal-2 increased and HA may be degenerated to lower MW. In some RA cases have high activity of Hyal in synovial fluid. HA with low MW may cause dysfunction of synovial fluid as lubricant and it also has proinflammatory effects, so the balance between HAS1, 2, 3 or Hyals may be important to maintain the joint's function normal.