The role of SDF-1α/CXCR4 signal in the lymph node metastases of early colon cancer.

本研究的目的：低侵袭性的内镜下切除术有望成为早期结肠癌患者的治疗手段。然而，他们已经被手术切除，直到现在，虽然淋巴结转移的风险小于10%。如果能阐明结肠癌的淋巴结转移机制，建立预测淋巴结转移的指标，则无淋巴结转移的早期结肠癌患者有可能通过内镜下低侵袭性切除术治愈。因此，本研究通过比较CXCR 4及其配体SDF-1α的表达与临床病理因素的关系，阐明CXCR 4及其配体SDF-1α是否是预测早期结肠癌淋巴结转移的有用指标。CXCR 4和SDF-1α在原发性SLE中的表达定位 关于我们 从结肠癌患者提供的手术切除的样品的离子进行了化学发光检查。共表达因子的检测：检测缺氧诱导因子（Hypoxia inducible factor，HIF）在缺氧条件下使CXCR 4过表达，同时检测作为基底膜成分之一的层粘连蛋白5γ 2链（Laminin-5γ 2 chain）的共表达。肿瘤细胞从肿瘤巢中分离的能力、迁移和粘附能力与CXCR 4/SDF-1α信号相关。与迁移和粘附能力的相关性：检查使用表达载体转染的CXCR 4过表达的结肠癌细胞是否影响迁移和粘附能力。类似地，考虑通过siRNA减少CXCR 4的细胞是否影响这些现象。两种因子的表达检查单层结肠癌细胞和类胶质形成细胞之间的差异。少

The purpose of this studyThe endoscopic resection with a low aggressiveness is expected from the treatment for patients of early colon cancer. However, they have been removed surgically until now though the risk of lymph node metastases are less than 10%. If the lymph node metastatic mechanism of colon cancer is elucidated and the marker to predict lymph node metastases is established, the patients of early colon cancer without lymph node metastasis might be cured by the endoscopic resection with low aggressiveness. Therefore, comparing between the expression of CXCR4 and of its ligand SDF-1α and the clinicopathological factorsin this study, I clarify whether both factors are an useful marker to predict the lymph node metastases in the early colon cancer.The study designHistopathological examination about the expression of CXCR4 and SDF-1α in the colon cancer tissue.Localization of the expression in the tumor tissue:Localization of the expression for CXCR4 and SDF-1α in the primary les … More ion of surgically resected specimens provided from colon cancer patients was immunohistochemically examined. The expression of both factors was compared about the correlation with lymph node metastases.The examination for coexpressed factors:The coexpression of Hypoxia inducible factor(HIF) to let overexpression of CXCR4 in hypoxic condition and Laminin-5γ2chain to be one of the basement membrane components were examined. The ability to dissociate from the neoplastic nest, the migration and the adhesion ability of tumor cells correlated with CXCR4/SDF-1αsignaling.Correlation with the ability of migration and adhesion:It was examined whether the CXCR4 overexpression colon cancer cells transfected using the expression vector affected the ability of migration and adhesion.Similarly, it was considered whether the CXCR4 reduced cells by siRNA affect these phenomenon.The expression of both factors examined the differences between the single layered colon cancer cells and the sphroid formed cells. Less