Determination of biochemical characteristics for the host-defense factors, APOBEC 3, to reveal the mechanisms of anti-retroelement activities

点击翻译按钮获取中文摘要

The human APOBEC3 family(A, B, C, DE, F, G, and H) proteins are cellular defense factors that potently inhibit the replication of retroviruses, including HIV. In order to clarify the anti-viral mechanism, we determined the biochemical characteristics of the enzymes. Furthermore, we identified four ubiquitination sites of APOBEC3G that are critical for HIV Vif-mediated degradation. Our findings will provide important informationto further understand the molecular mechanisms of Anti-viral activities as well as to develop attractive strategies utilizing potential endogenous inhibitors against HIV.