Studies on the early cancer detection with urinary diacetylspermine and its application

癌症患者尿液中N^1，N^N^&lt；12-二乙酰精胺(DiAcSpm)含量在疾病早期经常升高，因此被认为是很有前途的肿瘤标志物。在本研究中，我们首先对健康人的尿DiAcSpm水平进行了详细的分析，并建立了区分正常和异常的DiAcSpm排泄水平的临界值。然后，我们开发了一种免疫层析装置，用于简单快速地检测DiAcSpm。这一简单的检测程序的推广可能有助于增加癌症患者的早期发现机会。我们的进一步分析显示，DiAcSpm在癌症发展的早期阶段就在癌症组织中增加。这一发现一方面为我们提供了一种新的癌症早期检测方法，也为回答早期癌症患者尿液中DiAcSpm增加的问题提供了有用的线索。

N^1, N^<12>-diacetylspermine(DiAcSpm) in urine is frequently increased in cancer patients at earlier stages of the disease and hence is considered as promising tumor marker. In the present study we first analyzed the urinary DiAcSpm level of healthy persons in detail, and established cut-off values to distinguish between normal and abnormal levels of DiAcSpm excretion. We then developed an immunochromatographic device for simple and rapid DiAcSpm determination. The spreading of this simple determination procedure may help in increasing the opportunity of early detection of cancer patients. Our further analysis revealed that DiAcSpm is increased in cancer tissues from very early stages of cancer development. This finding provides us with a new method for early cancer detection on one hand, and a useful clue to answer the question of why DiAcSpm is increased in the urine from early cancer patients.