Identification of Female-Specific Transcriptional Factors for Osteopontin Translation as a Host Factor to Determine the Difference of Clinical Features of Liver Diseases between Male and Female Patients

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Osteopontin promoter SNPs(nt-155,-433) were responsible for the development of sexual difference of carcinogenesis in the liver. Promoter assay using male HepG2 cells and female MCF-7 cells revealed that the activities differed between alleles of either of SNPs in both cells. Also, gel-shift assay with nuclear extracts from both cells showed 3 signals ; a common signal for both cells, male-specific SRY and a female-specific indeterminate signal. The intensity of a common signal was different between the allele of nt-155, and that of a female-specific signal was increased following addition of estrogen to MCF-7 cells. These observations suggest that sexual difference of carcinogenesis may develop through these transcriptional factors which play differently between male and female cells.