Establishment of a simple differentiation diagnostic method of glucoprotein tumor markers by the pattern analysis in Western blotting

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It is not clear whether structures of glucoprotein tumor markers such carcinoembryonic antigen (CEA) and CA19-9 differ or not in various cancers. Western blot analysis of the expression patterns in various cancers was performed using original antibody against CEA which we made. The expression pattern of them was ladder bands pattern. In serum from lung, bands larger than 150kD were observed, but they were not in serum form other cancer patients and normal control (p=0.0352). Expression patterns were evaluated in such analysis using antibody against CA19-9 in sera from pancreas and colon cancers. A clue of simple differentiation diagnostic method of glucoprotein tumor markers by the pattern analysis in Western blotting was established.