Establishment of a simple differentiation diagnostic method of glucoprotein tumor markers by the pattern analysis in Western blotting

Western blotting模式分析建立糖蛋白肿瘤标志物简易鉴别诊断方法

• 批准号: 21590981
• 负责人: SATOH Hiroaki
• 金额: $ 3万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590981/
• 关键词: 癌; 肺癌; 腫瘍マーカー; 診断法; 泳動パターン; 診断; 糖鎖抗原; 血清診断

It is not clear whether structures of glucoprotein tumor markers such carcinoembryonic antigen (CEA) and CA19-9 differ or not in various cancers. Western blot analysis of the expression patterns in various cancers was performed using original antibody against CEA which we made. The expression pattern of them was ladder bands pattern. In serum from lung, bands larger than 150kD were observed, but they were not in serum form other cancer patients and normal control (p=0.0352). Expression patterns were evaluated in such analysis using antibody against CA19-9 in sera from pancreas and colon cancers. A clue of simple differentiation diagnostic method of glucoprotein tumor markers by the pattern analysis in Western blotting was established.

目前尚不清楚糖蛋白肿瘤标志物如癌胚抗原（CEA）和CA 19 -9的结构是否在各种癌症中不同。使用我们制备的抗CEA的原始抗体对各种癌症中的表达模式进行Western印迹分析。它们的表达呈梯状条带。在肺癌患者血清中，可观察到大于150 kD的条带，而在其他癌症患者和正常人血清中均未观察到（p=0.0352）。在这样的分析中，使用来自胰腺癌和结肠癌的血清中的抗CA 19 -9的抗体评价表达模式。建立了糖蛋白类肿瘤标志物Western印迹模式分析的简易鉴别诊断方法。

项目成果

泳動パターン解析による糖蛋白腫瘍マーカーの簡便な鑑別診断法の確立

利用迁移模式分析建立简单的糖蛋白肿瘤标志物鉴别诊断方法

• 发表时间: 2011
• 作者: 佐藤浩昭;他
• 通讯作者: 他