The investigation of the effect of transplantation of neural crest derived cells to murine focal cerebral ischemia model

神经嵴来源细胞移植对小鼠局灶性脑缺血模型的影响研究

• 批准号: 21591119
• 负责人: TOMITA Yutaka
• 金额: $ 2.83万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591119/
• 关键词: 神経堤細胞; 脳梗塞; Confocal顕微鏡; Matlab software; Matlab Software

The aim of the study was to monitor in vivo and noninvasively the fate of neural crest cells transplanted into the ischemic brain of unirradiated mice. In vivo imaging was performed through a closed cranial window following cell transplantation, using laser-scanning confocal fluorescence microscopy. Repeated in vivo imaging revealed that transplanted cells survived within the ischemic and peri-ischemic cortex, migrated significantly towards the lesion, proliferated. This study indicates that confocal fluorescence microscopy is a reliable and unique tool to repeatedly assess with cellular resolution the in vivo dynamic fate of fluorescent cells transplanted into a mouse brain.

该研究的目的是在体内以非侵入性方式监测移植到未受辐射小鼠缺血大脑中的神经嵴细胞的命运。使用激光扫描共焦荧光显微镜，通过细胞移植后的闭合颅窗进行体内成像。重复的体内成像显示，移植的细胞在缺血和缺血周围皮质内存活，显着向病灶迁移，并增殖。这项研究表明，共焦荧光显微镜是一种可靠且独特的工具，可以用细胞分辨率反复评估移植到小鼠大脑中的荧光细胞的体内动态命运。

项目成果

When does angiogenesis accompanied with RBC flow develop in ischemit tissue after permanent MCA occlusion in C57BL/6J mice?

C57BL/6J 小鼠永久 MCA 闭塞后，缺血组织中何时出现伴随红细胞流动的血管生成？

• 发表时间: 2009
• 期刊: J.Cereb.Blood Flow Metab. 29
• 作者: Tomita M;Tatarishvili J;Toriumi H;Tomita Y;Unekawa M;Hattori H;Suzuki N
• 通讯作者: Suzuki N

Variation of red blood cell velocities in intraparenchymal capillaries on potassium-induced cortical spreading depression in anesthetized rats

麻醉大鼠脑实质内毛细血管红细胞速度的变化对钾诱导的皮质扩散抑制的影响

• 发表时间: 2010
• 作者: 畝川美悠紀;冨田稔;冨田裕;鳥海春樹;鈴木則宏
• 通讯作者: 鈴木則宏

Artificial RBCs Transiently improved microcirculatory derangement produced by MCA occlusion or hemorrhagic hypotension in mice

人工红细胞暂时改善小鼠MCA闭塞或出血性低血压引起的微循环紊乱

• 发表时间: 2009
• 作者: Tomita Y;Toriumi H;Hattori H;Unekawa M;Tomita M;Itoh Y;Hoshino H;Sakai H;Tsuchida E;Horinouchi H;Kobayashi K;Suzuki N
• 通讯作者: Suzuki N

脳虚血周辺部微小血管の血管新生-経時的形態的変化と赤血球流速よりみたアプローチ脳虚血周辺部微小血管の血管新生-経時的形熊的変化と赤血球流速よりみたアプローチ

脑缺血周围微血管的血管生成 - 一种基于形态随时间和红细胞流速变化的方法 脑缺血周围微血管的血管生成 - 一种基于形态随时间和红细胞流速变化的方法

• 发表时间: 2009
• 作者: 冨田稔;Tatarishvili J;鳥海春樹;冨田裕;畝川美悠紀;服部英典;鈴木則宏
• 通讯作者: 鈴木則宏

RBC velocities in single capillaries of mouse and rat brains are the same, despite 10-fold difference in body size

• DOI: 10.1016/j.brainres.2010.01.032
• 发表时间: 2010-03-12
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Unekawa, Miyuki;Tomita, Minoru;Suzuki, Norihiro
• 通讯作者: Suzuki, Norihiro