A whole genomic analysis of acute myeloid leukemia.

急性髓系白血病的全基因组分析。

• 批准号: 21591217
• 负责人: YAMASHITA Yoshihiro
• 金额: $ 2.91万
• 依托单位: Jichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591217/
• 关键词: 急性骨髄性白血病; Blast Bank; ゲノムリシークエンス; 染色体構造異常; miRNA; 体細胞変異; アレイCGH

Leukemias are clonal disorders of hematopoietic stem cells or immature progenitors. However, a simple comparison of the bone marrow(BM) mononuclear cells(MNCs) among heterogeneous acute myeloid leukemia(AML) patients is likely to reveal a large number of changes in the genome that reflect differences either in the percentage of blasts within BM, or in the differentiation ability of the blasts. To minimize such population-shift effects, we have set up a large-scale cell bank(the Blast Bank) for the storage of hematopoietic stem cell(HSC)-like fractions purified from individuals with a wide range of leukemic disorders. To identify oncogenes in leukemias, and to clarify a molecular signature that was directly linked to the prognosis of leukemic patients, we performed a large-scale genomic analysis of the Blast Bank specimens.

白血病是造血干细胞或未成熟祖细胞的克隆性疾病。然而，对异质急性髓系白血病（AML）患者的骨髓（BM）单核细胞（MNC）进行简单比较可能会揭示基因组中的大量变化，这些变化反映了BM内原始细胞百分比或原始细胞分化能力的差异。为了最大限度地减少这种群体转移效应，我们建立了一个大规模细胞库（Blast Bank），用于储存从患有多种白血病疾病的个体中纯化的造血干细胞（HSC）样组分。为了鉴定白血病中的癌基因，并澄清与白血病患者预后直接相关的分子特征，我们对 Blast Bank 样本进行了大规模基因组分析。

项目成果

Screening for genetic abnormalities involved in ovarian carcinogenesis using retroviral expression libraries

• DOI: 10.3892/ijo_00000410
• 发表时间: 2009-11-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Wada, Tomoaki;Yamashita, Yoshihiro;Suzuki, Mitsuaki
• 通讯作者: Suzuki, Mitsuaki

KIF5B-ALK, a Novel Fusion Oncokinase Identified by an Immunohistochemistry-based Diagnostic System for ALK-positive Lung Cancer

• DOI: 10.1158/1078-0432.ccr-08-3248
• 发表时间: 2009-05-01
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Takeuchi, Kengo;Choi, Young Lim;Mano, Hiroyuki
• 通讯作者: Mano, Hiroyuki

Array-based genomic resequencing of acute myeloid leukemia.

基于芯片的急性髓系白血病基因组重测序。

• 发表时间: 2010
• 作者: 平野育生、峯岸直子;他;山下義博
• 通讯作者: 山下義博

Array-based genomic resequencing of human leukemia

• DOI: 10.1038/onc.2010.117
• 发表时间: 2010-06-24
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Yamashita, Y.;Yuan, J.;Mano, H.
• 通讯作者: Mano, H.

A large-scale resequencing project of ~5600 human genes in the genome of acute myeloid leukemia.

急性髓系白血病基因组中约 5600 个人类基因的大规模重测序项目。

• 发表时间: 2009
• 作者: Yamashita;Y.;Soda;M.;Mano;H.
• 通讯作者: H.