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The study about control of acute inflammation period after free flap transplantation using transfection of Decoy.

利用Decoy转染控制游离皮瓣移植后急性炎症期的研究。

基本信息

批准号：
16592014
负责人：
YAMASHITA Yoshihiro
金额：
$ 2.24万
依托单位：
Kyushu Dental Collage
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

This research controlled the transcript factor by using the decoy gene, and examined the revitalization of transcript factor NF-kB in which it took part in the appearance of an inflammatory cytokine which controlled result of the vascularized tissue transplant by the rat with the blood vessel handle about overcoming the acute condition period after the art of rebuilding of the separation organization transplant with the blood vessel handle. Decoy ODNs content HVJ-ribosome is injected from the superficial abdominal wall artery of the abdomen separation dermal flap with the made rat blood vessel handle, and Decoy ODNs is introduced in the dermal flap organization.The appearance of IL-6 in the Northern bloth method which used screening by the immunity histochemistry dye and the organization extraction thing and IL-8 was retrieved.The significant difference between each crowd was not admitted in the appearance of IL-6 in the Northern bloth method and IL-8 though the tendency to which the inflammatory cell permeation in the dermal flap surroundings was weak was admitted in the crowd which administered NF-kB Decoy ODNs content HVJ-ribosome to the free flap.Though the appearance of two or more, inflammatory cytokines is controlled by introducing it while organizing the decoy gene of NF-kB with the above-mentioned result, and the possibility of overcoming an inflammation period was suggested, it seemed that it was necessary to examine the administering density and the administering method of Decoy ODNs content HVJ-ribosome further.

本研究利用诱导性基因控制转录因子，研究了转录因子NF-kB的活化过程，其中NF-kB参与了一种炎症细胞因子的出现，这种炎症细胞因子控制血管柄移植大鼠血管化组织移植后重建分离组织的急性期。用自制的大鼠血管柄，从腹部分离真皮皮瓣的腹壁浅动脉注入诱饵ODNs含量hvj -核糖体，并在真皮皮瓣组织中引入诱饵ODNs。检索了免疫组化染色和组织提取物筛选的Northern bloth法中IL-6和IL-8的出现情况。Northern bloth法检测的IL-6和IL-8的出现不存在显著性差异，但在给予游离皮瓣NF-kB Decoy ODNs含量hvj核糖体的人群中存在炎症细胞在皮瓣周围渗透较弱的趋势。虽然两种或两种以上的炎症因子的出现是通过在组织NF-kB诱饵基因的同时引入其来控制的，并通过上述结果提示克服炎症期的可能性，但似乎有必要进一步研究诱饵ODNs含量hvj核糖体的给药密度和给药方法。