The study about control of acute inflammation period after free flap transplantation using transfection of Decoy.

本研究通过使用诱饵基因控制转录因子，并检查转录因子NF-κ B的复活，其中NF-κ B参与了控制带血管柄的大鼠血管化组织移植结果的炎性细胞因子的出现，其克服了带血管柄的分离组织移植重建术后的急性状态期。将含有诱饵ODNs的HVJ-核糖体经制作好的大鼠血管手柄从腹部分离皮瓣的腹壁浅动脉注入，采用免疫组化染色和组织提取物筛选的北方印迹法检测IL-6和IL-10的表达，在北方印迹法中，IL-6的出现在各组之间没有显著差异，而IL-8在施用NF-κ B的组中，尽管在皮瓣周围炎性细胞渗透较弱的趋势被承认。虽然上述结果表明，在组织NF-κ B诱饵基因的同时引入两种或两种以上的炎症细胞因子是可以控制的，并且提出了克服炎症期的可能性，但似乎有必要进一步检查含HVJ-核糖体的诱饵ODNs的施用浓度和施用方法。

This research controlled the transcript factor by using the decoy gene, and examined the revitalization of transcript factor NF-kB in which it took part in the appearance of an inflammatory cytokine which controlled result of the vascularized tissue transplant by the rat with the blood vessel handle about overcoming the acute condition period after the art of rebuilding of the separation organization transplant with the blood vessel handle. Decoy ODNs content HVJ-ribosome is injected from the superficial abdominal wall artery of the abdomen separation dermal flap with the made rat blood vessel handle, and Decoy ODNs is introduced in the dermal flap organization.The appearance of IL-6 in the Northern bloth method which used screening by the immunity histochemistry dye and the organization extraction thing and IL-8 was retrieved.The significant difference between each crowd was not admitted in the appearance of IL-6 in the Northern bloth method and IL-8 though the tendency to which the inflammatory cell permeation in the dermal flap surroundings was weak was admitted in the crowd which administered NF-kB Decoy ODNs content HVJ-ribosome to the free flap.Though the appearance of two or more, inflammatory cytokines is controlled by introducing it while organizing the decoy gene of NF-kB with the above-mentioned result, and the possibility of overcoming an inflammation period was suggested, it seemed that it was necessary to examine the administering density and the administering method of Decoy ODNs content HVJ-ribosome further.