Biological and functional analysis of SFRP1, a novel regulator on early process of lymphopoiesis

淋巴细胞生成早期过程的新型调节因子 SFRP1 的生物学和功能分析

• 批准号: 21591241
• 负责人: YOKOTA Takafumi
• 金额: $ 2.91万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591241/
• 关键词: 血液免疫学; リンパ球前駆細胞; 造血幹細胞; Wntシグナル; sFRP; SFRP1; SFRP5

We reported that early lymphopoiesis is suspended during pregnant period and high-estrogen condition. By means of intensive analyses on the influence of estrogen on the hematopoietic microenvironment, we have found that soluble Frizzled-related protein 1(SFRP1) acts as a mediator of estrogen action. In the present study, we have constructed transgenic mouse models of SFRP1 or other SFRP-related molecules to evaluate their biological significance in vivo. We have observed severe leukocytopenia, particularly decrease of B-lymphocytes, in SFRP over-expressing mice.

我们报道了妊娠期和高雌激素状态下，早期淋巴细胞生成暂停。通过深入分析雌激素对造血微环境的影响，我们发现可溶性卷曲相关蛋白1（SFRP 1）作为雌激素作用的介质。在本研究中，我们构建了SFRP 1或其他SFRP相关分子的转基因小鼠模型，以评估其在体内的生物学意义。我们已经观察到严重的白细胞减少症，特别是B淋巴细胞减少，在SFRP过表达的小鼠。

项目成果

Deletion of core fucosylation on pre-BCR down-regulates its functions

BCR前体核心岩藻糖基化的缺失会下调其功能

• 发表时间: 2010
• 作者: Li W;Wang H;Liu Q;Ishihara K;Yokota T;Gu J;Taniguchi N;Kondo A.
• 通讯作者: Kondo A.

The endothelial antigen ESAM marks hematopoietic stem/progenitor cells throughout ontogeny in mice.

内皮抗原 ESAM 在小鼠的整个个体发育过程中标记造血干/祖细胞。

• 发表时间: 2009
• 作者: Ishiko E;et al.;Kanakura Y.;Yokota T.
• 通讯作者: Yokota T.

SATB1 Induces Early Lymphocyte Differentiation From Primitive Hematopoietic Progenitors

SATB1 诱导原始造血祖细胞早期淋巴细胞分化

• 发表时间: 2011
• 作者: Satoh Y;Yokota T;Tanaka H;Kokame K;Miyata T;Matsumura I;Oritani K;Kanakura Y
• 通讯作者: Kanakura Y

Satb1 promotes hematopoietic stem cell differentiation toward the lymphoid lineages

Satb1促进造血干细胞向淋巴谱系分化

• 发表时间: 2011
• 作者: Satoh Y;Yokota T;Kondo M;Lai A;Kincade P;Kouro T;Iida R;Kokame K;Miyata T;Sudo T;Tanaka H;Matsumura I;Oritani K;Kohwi-Shigematsu T;Kanakura Y
• 通讯作者: Kanakura Y

Satb1 directs hematopoietic stem cell differentiation toward the lymphoid lineages

Satb1 引导造血干细胞向淋巴谱系分化

• 发表时间: 2011
• 作者: Yokota T;Satoh Y;Kondo M;Kincade PW;Kouro T;Iida R;Sudo T;Tanaka H;Matsumura I;Oritani K;Kohwi-Shigematsu T;Kanakura Y.
• 通讯作者: Kanakura Y.