Research on aging of human hematopoietic stem cells

人类造血干细胞衰老的研究

• 批准号: 21591367
• 负责人: KAWANO Yoshifumi
• 金额: $ 2.83万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591367/
• 关键词: 造血幹細胞; 造血幹細胞移植; CD34陽性細胞; 加齢; 老化; 転写因子; ヒト造血幹細胞; 造血細胞移植術; 加齢現象; IKAROS; CEPBPAα; リンパ球/骨髄球比; 造血細胞移植; リンパ球比率

To investigate aging of human hematopoietic stem cells, we evaluated the expression levels The median age of the donors was 21 y(range 0.2-44y). The purity of CD34-positive cells was greater than 90%. Both target genes were quantitatively detected in all samples. There was an inverse correlation between the expression level of ikzf1 and the donor's age(r=-0.358, p=0.032). In contrast, there was no correlation between the expression level of cebpa and the donor's age. From the data, the mRNA level of ikzf1 in purified CD34-positive cells may reflect the age of HSCs in human bone marrow.

为了研究人造血干细胞的老化，我们评估了表达水平。供体的中位年龄为21岁（范围0.2- 44岁）。CD 34阳性细胞的纯度大于90%。在所有样品中定量检测到两种靶基因。IKZF 1的表达水平与供者年龄呈负相关（r=-0.358，p =0.032）。与此相反，cebpa的表达水平与供者年龄无关。从数据来看，纯化的CD 34阳性细胞中ikzf 1的mRNA水平可以反映人骨髓中HSC的年龄。

项目成果

Lymphoid commitment in human bone marrow CD34-positive cells reflects aging of hematopoietic stem cells

人骨髓 CD34 阳性细胞中的淋巴定向反映了造血干细胞的衰老

• 发表时间: 2011
• 作者: Kodama Y;Miyazono A;Okamoto Y;Yamaki Y;Kurauchi K;Nishikawa T;Tanabe T;Shinkoda Y;Kawano Y.
• 通讯作者: Kawano Y.

HIF-1α is not associated with the clinical course of aplastic anemia in children

HIF-1α 与儿童再生障碍性贫血的临床病程无关

• 发表时间: 2011
• 作者: Kodama Y;Okamoto Y;Shinkoda Y;Tanabe T;Nishikawa T;Yamaki Y;Kawano Y.
• 通讯作者: Kawano Y.

Benzene metabolite 1,2,4-benzenetriol induces halogenated DNA and tyrosines representing halogenative stress in the HL-60 human myeloid cell line.

• DOI: 10.1289/ehp.1103437
• 发表时间: 2012-01
• 期刊: Environmental health perspectives
• 影响因子: 10.4
• 作者: Nishikawa T;Miyahara E;Horiuchi M;Izumo K;Okamoto Y;Kawai Y;Kawano Y;Takeuchi T
• 通讯作者: Takeuchi T

Myeloperoxidase-derived halogenative stress, a possible cause of benzene leukemogenesis

髓过氧化物酶衍生的卤化应激，苯白血病发生的可能原因

• 发表时间: 2011
• 作者: Nishikawa T;Izumo K;Miyahara E;Horiuchi M;Okamoto Y;Kawai Y;Kawano Y;Takeuchi T.
• 通讯作者: Takeuchi T.

Successful bone marrow transplantation for children with aplastic anemia based on a best-available evidence strategy

• DOI: 10.1111/j.1399-3046.2010.01388.x
• 发表时间: 2010-12-01
• 期刊: PEDIATRIC TRANSPLANTATION
• 影响因子: 1.3
• 作者: Okamoto, Yasuhiro;Kodama, Yuichi;Kawano, Yoshifumi
• 通讯作者: Kawano, Yoshifumi