Involvement of aquaporin-3 in keratinocyte proliferation and tumorigenesis

水通道蛋白-3 参与角质形成细胞增殖和肿瘤发生

• 批准号: 21591463
• 负责人: CHIKUMA Mariko
• 金额: $ 2.91万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591463/
• 关键词: 皮膚科学; アクアポリン; 細胞増殖; アトピー性皮膚炎

We investigated the involvement of aquaporin-3(AQP3) in epidermalhyperplasia in skin diseases, such as atopic dermatitis(AD). We found significantly increasedAQP3 transcript and protein expression in the epidermis of human AD lesions. The upregulation ofAQP3 expression in human keratinocytes by transfection with human AQP3 DNA plasmid increased cell proliferation. In mouse AD models, AQP3 was strongly overexpressed in the epidermis in wild-type mice. Epidermal hyperplasia was reduced in AQP3-deficient mice, with a decreased number of proliferating keratinocytes and normal barrier function. These results suggest the involvement of AQP3 in epidermal hyperplasia by a mechanism involving upregulated AQP3 expression and consequent enhancement of keratinocyte proliferation.

我们研究了水通道蛋白-3（AQP3）在皮肤疾病（如特应性皮炎（AD））表皮增生中的作用。我们发现在人AD病变的表皮中aqp3转录物和蛋白表达显著增加。转染人AQP3 DNA质粒可上调人角质形成细胞AQP3的表达，促进细胞增殖。在小鼠AD模型中，AQP3在野生型小鼠表皮中强烈过表达。aqp3缺陷小鼠表皮增生减少，增殖角化细胞数量减少，屏障功能正常。这些结果表明，AQP3参与表皮增生的机制涉及AQP3表达上调和角化细胞增殖的增强。

项目成果

Acontrolled aquaporin-3 expression in T lymphocytes regulatetheir migration and trafficking in cutaneous immune reaction

T淋巴细胞中受控的水通道蛋白3表达调节其在皮肤免疫反应中的迁移和运输

• 发表时间: 2010
• 作者: 竹馬真理子、竹馬俊介、椛島健治、AS.Verkman;杉山義宣、井上紳太郎、宮地良樹
• 通讯作者: 杉山義宣、井上紳太郎、宮地良樹

The expression of differentiation markers in aquaporin-3 deficient epidermis.

• DOI: 10.1007/s00403-009-0927-9
• 发表时间: 2009-03
• 期刊: Archives of dermatological research
• 影响因子: 3
• 作者: Hara-Chikuma M;Takahashi K;Chikuma S;Verkman AS;Miyachi Y
• 通讯作者: Miyachi Y

Upregulation of Aquaporin-3 Is Involved in Keratinocyte Proliferation and Epidermal Hyperplasia

• DOI: 10.1038/jid.2010.395
• 发表时间: 2011-04-01
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Nakahigashi, Kyoko;Kabashima, Kenji;Hara-Chikuma, Mariko
• 通讯作者: Hara-Chikuma, Mariko