Involvement of aquaporin-3 in keratinocyte proliferation and tumorigenesis

我们研究了水通道蛋白-3（AQP3）在皮肤疾病（如特应性皮炎（AD））表皮增生中的作用。我们发现在人AD病变的表皮中aqp3转录物和蛋白表达显著增加。转染人AQP3 DNA质粒可上调人角质形成细胞AQP3的表达，促进细胞增殖。在小鼠AD模型中，AQP3在野生型小鼠表皮中强烈过表达。aqp3缺陷小鼠表皮增生减少，增殖角化细胞数量减少，屏障功能正常。这些结果表明，AQP3参与表皮增生的机制涉及AQP3表达上调和角化细胞增殖的增强。

We investigated the involvement of aquaporin-3(AQP3) in epidermalhyperplasia in skin diseases, such as atopic dermatitis(AD). We found significantly increasedAQP3 transcript and protein expression in the epidermis of human AD lesions. The upregulation ofAQP3 expression in human keratinocytes by transfection with human AQP3 DNA plasmid increased cell proliferation. In mouse AD models, AQP3 was strongly overexpressed in the epidermis in wild-type mice. Epidermal hyperplasia was reduced in AQP3-deficient mice, with a decreased number of proliferating keratinocytes and normal barrier function. These results suggest the involvement of AQP3 in epidermal hyperplasia by a mechanism involving upregulated AQP3 expression and consequent enhancement of keratinocyte proliferation.