Identification of biomarkers for differential diagnosis of neurodegenerative and non-neurodegenerative dementia

阿尔茨海默病（AD）与其他形式的痴呆不同，它与淀粉样蛋白β肽（a β_<42>）有关，淀粉样蛋白β肽对神经元细胞具有毒性作用。这表明a - β42改变了神经元细胞中的蛋白表达。本研究采用小鼠原代培养神经元，研究了Aβ_<42>和不加Aβ_<42>孵育后上清液中蛋白质的变化。鉴于凋亡细胞外质膜上酸性磷脂（磷脂酰丝氨酸（PS））的出现，我们使用PS作为探针，并使用蛋白质组学方法鉴定了与PS包被的磁性纳米珠结合的蛋白质。在已确定的许多蛋白中，我们重点研究了膜联蛋白A5和乳脂球egf因子8(MFG-E8)，它们参与凋亡细胞的清除。a β <42>的培养上清中膜联蛋白A5和MFG-E8均显著升高。

Alzheimer's disease(AD) differs from other forms of dementia in its relation to amyloid beta peptide(Aβ_<42>), which has a toxic effect on neuronal cells. This effect implies that protein expression is changed in neuronal cells by Aβ42. In the present study, we used the mice primary culture neurons and investigated the proteins in the supernatant after incubation with or without Aβ_<42>. In view of the appearance of an acidic phospholipid(phosphatidylserine(PS)) on the outer plasma membrane of an apoptotic cell, we used PS as a probe and proteins bound to PS-coated magnetic nano-beads were identified using a proteomic approach. Of a number of proteins identified, we focused on annexin A5 and milk fat globule-EGF-factor 8(MFG-E8) that is involved in the clearance of apoptotic cells. Both annexin A5 and MFG-E8 were found to be increased significantly in the culture supernatant by Aβ_<42>.