Determination of pathogenesis and therapeutic agents in gastric cancer patient with disseminated carcinomatosis of bone marrow

胃癌伴骨髓播散癌的发病机制及治疗药物的确定

• 批准号: 21591703
• 负责人: HAYASHI Naoko
• 金额: $ 2.91万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591703/
• 关键词: 癌幹細胞; 胃癌骨髄癌腫症; 遊離癌細胞(ITC)

We isolated circulating tumor cells(CTCs) using CellSearch^<TM> in five cases of gastric cancer patients with disseminated carcinomatosis of bone marrow. Then, we could identify several candidate genes involved in pathogenesis of this disease, based on the result of gene expression profiling of collected CTCs. As CTCs in two patients remarkably increased and most of CTCs highly expressed HER2, we used' trastuzumab', the monoclonal antibody against HER2, combined with the chemotherapy, against these two patients. They had remission of DIC and long-term survival. Moreover, we could establish mouse xenograft model using CTCs obtained from two gastric cancer patients with disseminated carcinomatosis of bone marrow.

我们使用CellSearch^<TM>在5例伴有骨髓播散性癌病的胃癌患者中分离循环肿瘤细胞（CTC）。然后，我们可以确定几个候选基因参与这种疾病的发病机制，根据基因表达谱的结果收集CTC。由于两名患者的CTC显著增加，并且大多数CTC高度表达HER2，我们使用抗HER2单克隆抗体“曲妥珠单抗”与化疗联合治疗这两名患者。他们有DIC缓解和长期生存。此外，我们可以建立小鼠异种移植模型，从两个胃癌患者的骨髓播散性癌病的CTC。

项目成果

Adenoviral oncolytic suicide gene therapy for a peritoneal dissemination model of gastric cancer in mice.

腺病毒溶瘤自杀基因治疗小鼠胃癌腹膜传播模型。

• 发表时间: 2010
• 期刊: Ann Surg Oncol.
• 作者: Amagane H;Watanabe Y;Kaneko N;Nunokawa A;Muratake T;Ishiguro H;Arinami T;Ujike H;Inada T;Iwata N;Kunugi H;Sasaki T;Hashimoto R;Itokawa M;Ozaki N;Someya T.;Kimura T;Imamura Yu
• 通讯作者: Imamura Yu

消化器癌における微量癌細胞の検出とHER2陽性微量癌細胞を標的とした個別化治療への応用

胃肠癌中痕量癌细胞的检测及其应用于针对HER2阳性痕量癌细胞的个体化治疗

• 发表时间: 2012
• 作者: 岩槻政晃;渡邊雅之;石本崇胤;岩上志朗;馬場祥史;林尚子;豊島功正;林哲;大田義一;馬場秀夫
• 通讯作者: 馬場秀夫

Laparoscopy assisted resection of an undiagnosed liver tumor and ascending colon cancer via mini median laparotomy : report of a case

腹腔镜辅助小正中剖腹手术切除未确诊的肝脏肿瘤和升结肠癌一例

• 发表时间: 2011
• 期刊: Surg Today
• 影响因子: 2.5
• 作者: Mima K;Beppu T;Chikamoto A;Ishiko T;Horino K;Hayashi N;Watanabe M;Takamori H;Okabe K;Yamanaka T;Baba H
• 通讯作者: Baba H

Usefulness of Transcription-Reverse Transcription Concerted Reaction Method for Detecting Circulating Tumor Cells in Patients With Colorectal Cancer

• DOI: 10.1245/s10434-011-1889-7
• 发表时间: 2012-06-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Sato, Nobutaka;Hayashi, Naoko;Baba, Hideo
• 通讯作者: Baba, Hideo

The central vein access port and catheter in outpatient chemotherapy for colorectal cancer : a retrospective study of 101 patients

中心静脉通路和导管在结直肠癌门诊化疗中的应用：101例患者的回顾性研究

• 发表时间: 2012
• 期刊: Surg Today
• 影响因子: 2.5
• 作者: Sawayama H;Hayashi N;Watanabe M;Takamori H;Beppu T;Baba H
• 通讯作者: Baba H