A next step study on candidate genes regulating metastasis of colorectal cancer

调控结直肠癌转移候选基因的下一步研究

• 批准号: 21591719
• 负责人: MIURA Koh
• 金额: $ 2.83万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591719/
• 关键词: 大腸癌; 転移

We are analyzing orthotopically-transplanted tumors of 22 types of colorectal cancer cell lines in mouse models on epithelial-to-mesenchymal transition. Firstly E-cadherin and vimentin were immunohistochemically analyzed, and contrasting results were obtained with two cell lines : HCT116 and HT29. Currently we are examining the results for 22 types of cell lines. Next, we are analyzing the expressions of RNF125, TCF25, CRIP1, LOXL3, EN2, P2RX5 and other genes, which are candidate genes to regulate lymph node metastasis, on their expressions with RT-PCR using clinical materials, whether the results are reproducible compared with those acquired with cell lines. It seems that the expressions of RNF125 and CRIP1 are higher in more advanced cases ; on the other hand, the expressions of LOXL3, EN2, and P2RX5 genes seems to be lower in more advanced cases although the results still have to be confirmed.

我们正在分析原位移植肿瘤的22种类型的结直肠癌细胞系在小鼠模型上皮细胞间质转化。首先对E-cadherin和vimentin进行了免疫组化分析，并与两种细胞系HCT 116和HT 29的结果进行了对比。目前，我们正在研究22种细胞系的结果。接下来，我们使用临床材料，通过RT-PCR分析作为调节淋巴结转移的候选基因的RNF 125、TCF 25、CRIP 1、LOXL 3、EN 2、P2 RX5和其他基因的表达，与用细胞系获得的结果相比，分析结果是否可重复。RNF 125和CRIP 1的表达似乎在更晚期的病例中更高;另一方面，LOXL 3、EN 2和P2 RX5基因的表达似乎在更晚期的病例中更低，尽管结果仍有待证实。

项目成果

siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis

• DOI: 10.1016/j.bbrc.2009.10.127
• 发表时间: 2009-12-25
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kaneko, Naoyuki;Miura, Koh;Horii, Akira
• 通讯作者: Horii, Akira

Interim Report

• DOI: 10.3327/jaesj.26.686
• 发表时间: 1996
• 作者: Magnus Ahlqvist President and Ceo;Andreas Lindback Cfo

Young-onset peri-anorectal leiomyomatosis: report of a case.

年轻发病的肛门直肠周围平滑肌瘤病：一例报告。

• DOI: 10.1007/s00595-012-0201-1
• 发表时间: 2012
• 期刊: Surg Today
• 影响因子: 2.5
• 作者: Toshima M;Miura K;Ishida K;Mizoi T;Naitoh T;Ogawa H;Ohnuma S;Sasaki H;Watanabe K;Haneda S;Sase T;Kajiwara T;Matsuda Y;Shibata C;Sasaki I.
• 通讯作者: Sasaki I.

A phase II multicenter trial of neoadjuvant chemotherapy FOLFOX6 in combination with bevacizumab for patients with resectable synchronous liver metastases after R0-resections of primary colorectal cancers : The interim analysis

新辅助化疗 FOLFOX6 联合贝伐单抗治疗原发性结直肠癌 R0 切除后可切除同时性肝转移患者的 II 期多中心试验：中期分析

• 发表时间: 2011
• 作者: Miura K;Katayose Y;Oikawa M;Yamauchi J;Sakurai N;Musha H;Narushima Y;Nakagawa K;Egawa S;Unno M.
• 通讯作者: Unno M.

消化器疾患における分子腫瘍マーカーの新展開大腸癌の5-FU感受性規定因子としてのcIAP2遺伝子およびIAPファミリー遺伝子群の研究とその臨床応用

胃肠道疾病分子肿瘤标志物新进展 cIAP2基因和IAP家族基因作为结直肠癌5-FU敏感性决定因素的研究及其临床应用

• 发表时间: 2010
• 作者: 三浦康;唐澤秀明;佐々木巖
• 通讯作者: 佐々木巖