Prediction of prognosis of hepatocellular carcinoma with use of measurement of methylated CCND2 gene in the blood

本研究的目的是建立一个微创的预测系统，使用甲基化的CCND2基因在血清中负责肝细胞癌（HCC）患者的结果。(1)确定了血清采集和DNA提取的条件。(2)建立了CCND2基因甲基化特异性定量PCR分析方法。(3)我们发现血清中CCND2基因甲基化水平与患者预后不良相关。6)在学术会议上宣读论文17篇

The purpose of this study is to develop a minimally invasive predictive system with use of methylated CCND2 gene in the serum responsible for outcome of patients with hepatocellular carcinoma(HCC).(1) We determined several conditions for serum collection and DNA extraction.(2) We established a quantitative methylation-specific PCR analysis for CCND2 gene in serum.(3) We demonstrated that methylation levels of CCND2 gene in serum were associated with poor outcome of patentsWe reported the relevant research in the form of 9 papers(total of IF=26. 6) and read 17 papers at academic meetings