Angiogenesis and lymphagenesis targeted molecular therapy for cholangiocarcinoma.

胆管癌的血管生成和淋巴生成靶向分子治疗。

• 批准号: 21591765
• 负责人: HIRANO Tadamichi
• 金额: $ 2.83万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591765/
• 关键词: 肝臓外科学; CCC; ケモカイン; 分子治療; 新生血管抑制; アンタゴニスト

In the present study, we investigated the role of CXCR2 in intrahepatic cholangiocellular carcinoma(ICC). First, expression of CXCR2 is estimated by immunohistochemical staining in ICC using thirty ICC human specimens. Next, the role of CXCR2 was estimated in vitro by proliferation assay, migration assay, or invasion assay. In vivo effect was also estimated using xenograft mice model. As a result, blockage of CXCR2 significantly inhibited proliferation, migration, and invasion of ICC in vitro. Tumor growth of xenograft mice model was also inhibited by CXCR2 antagonist. Our results suggested that CXCR2 act crucial role in the development of ICC. Blockage of CXCR2 may be a promising therapeutic approach for ICC.

在本研究中，我们研究了CXCR2在肝内胆管细胞癌（ICC）中的作用。首先，使用三十个 ICC 人类样本，通过 ICC 中的免疫组织化学染色来估计 CXCR2 的表达。接下来，通过增殖测定、迁移测定或侵袭测定在体外评估CXCR2的作用。还使用异种移植小鼠模型评估了体内效果。结果，CXCR2的阻断显着抑制体外ICC的增殖、迁移和侵袭。 CXCR2拮抗剂也抑制异种移植小鼠模型的肿瘤生长。我们的结果表明 CXCR2 在 ICC 的发展中发挥着至关重要的作用。阻断 CXCR2 可能是 ICC 的一种有前途的治疗方法。

项目成果

Blockage of CXCR 2 suppresses cell proliferation, migration and invasion in intrahepatic cholangiocellular carcinoma

阻断 CXCR 2 可抑制肝内胆管细胞癌的细胞增殖、迁移和侵袭

• 发表时间: 2011
• 作者: H. Sueoka;T. Hirano;N. Uyama;U. Iimuro;T. Okada;J. Fujimoto
• 通讯作者: J. Fujimoto

Blockage of CXCR2 suppresses tumor growth of intrahepatic cholangiocellular carcinoma

• DOI: 10.1016/j.surg.2013.12.037
• 发表时间: 2014-04-01
• 期刊: SURGERY
• 影响因子: 3.8
• 作者: Sueoka, Hideaki;Hirano, Tadamichi;Fujimoto, Jiro
• 通讯作者: Fujimoto, Jiro