Angiogenesis and lymphagenesis targeted molecular therapy for cholangiocarcinoma.

在本研究中，我们探讨了CXCR2在肝内胆管细胞癌中的作用。首先，用免疫组织化学方法检测30例ICC标本中CXCR2的表达。接下来，通过增殖实验、迁移实验或侵袭实验来评估CXCR2在体外的作用。用异种移植小鼠模型评估体内效应。结果：阻断CXCR2可显著抑制ICC的体外增殖、迁移和侵袭。CXCR2拮抗剂对移植瘤小鼠的肿瘤生长也有抑制作用。我们的结果提示CXCR2在ICC的发生发展中起着重要作用。阻断CXCR2可能是治疗ICC的一种有前途的治疗方法。

In the present study, we investigated the role of CXCR2 in intrahepatic cholangiocellular carcinoma(ICC). First, expression of CXCR2 is estimated by immunohistochemical staining in ICC using thirty ICC human specimens. Next, the role of CXCR2 was estimated in vitro by proliferation assay, migration assay, or invasion assay. In vivo effect was also estimated using xenograft mice model. As a result, blockage of CXCR2 significantly inhibited proliferation, migration, and invasion of ICC in vitro. Tumor growth of xenograft mice model was also inhibited by CXCR2 antagonist. Our results suggested that CXCR2 act crucial role in the development of ICC. Blockage of CXCR2 may be a promising therapeutic approach for ICC.