Detection of tyrosine kinase gene mutation using genotyping assay

利用下一代测序技术在肺癌中发现了新的KIF5B/RET易位。建立了检测KIF5B/RET输血基因的荧光原位杂交(FISH)方法。我们采用基因分型方法检测了Kras基因突变情况，并与肺癌组织中Kras基因拷贝数、GLUT-1表达等临床病理背景进行了比较。

Novel KIF5B/RET translocation was found in lung cancer using a next-generation sequencing assay. We have developed fluorescence in situ hybridization(FISH) assay to detect KIF5B/RET transfusion gene. We have investigated Kras gene mutation statuses using genotyping assay and compared with Kras copy number, Glut-1 expression and other clinico-pathological backgrounds of lung cancers.