The coadministration of granulocyte colony-stimulating factor and stem cell factor to secondary injury after spinal cord injury(Analysis of endplasmic reticulum stress response)

粒细胞集落刺激因子与干细胞因子联合应用对脊髓损伤后继发性损伤的影响（内质网应激反应分析）

• 批准号: 21591907
• 负责人: WATANABE Masahiko
• 金额: $ 3万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591907/
• 关键词: 脊椎脊髄病学; 脊髄損傷; 二次損傷; オリゴデンドロサイト前駆細胞; アポトーシス; 小胞体ストレス; GRP78; CHOP/GADD153; オリゴデンドロサイト; 再髄鞘化

In this study, We focused on endoplasmic reticulum(ER) stress as a cause of apoptosis of oligodendrocyte precursor cell(OPC) following spinal cord injury, and examined it in vivo, and in vitro. In addition, We investigated the effects of G-CSF and SCF on intrinsic cells, and motor function recovery in spinal cord-injured mice. The results showed that the combined administration of SCF and G-CSF in mouse contusive spinal cord injury model not only improved motor function, but also induced the accumulation of intrinsic microglia and the active proliferation of intrinsic OPC. Our results suggest that apoptosis by the ER stress is involved in the differential inhibition of OPC, and To increase the tolerance of ER stress response of OPC may result in remyelination of damaged spinal cord.

本研究旨在探讨内质网应激对脊髓损伤后少突胶质前体细胞凋亡的影响，并在体内外进行了研究。此外，我们还观察了G-CSF和SCF对脊髓损伤小鼠内源性细胞和运动功能恢复的影响。结果表明，SCF和G-CSF联合应用不仅能改善小鼠脊髓挫伤模型的运动功能，而且能诱导内源性小胶质细胞的聚集和内源性OPC的活跃增殖。结果提示，内质网应激引起的细胞凋亡参与了OPC的差异性抑制，增强OPC对内质网应激反应的耐受性可能导致受损脊髓的髓鞘再生。

项目成果

Response of Grp78 protein in glial cell line for cytotoxic factor by traumatic stress : an in vitro study

神经胶质细胞系中 Grp78 蛋白对创伤应激引起的细胞毒性因子的反应：一项体外研究

• 发表时间: 2009
• 作者: Suyama;K.
• 通讯作者: K.

Endlasmic reticulum stress response in the rat contusive spinal cord injury model

大鼠脊髓挫伤模型中的内质网应激反应

• 发表时间: 2011
• 作者: Daisuke Matsuyama;Masahiko Watanabe;Kaori Suyama;Masahiro Kuroiwa;Joji Mochida
• 通讯作者: Joji Mochida

ラット脊髄圧挫損傷モデルにおける小胞体ストレス応答の変化に関する組織学的検討

大鼠脊髓挤压伤模型内质网应激反应变化的组织学检查

• 发表时间: 2011
• 作者: 松山大輔;渡辺雅彦;隅山香織;黒岩真弘;持田讓治
• 通讯作者: 持田讓治