Molecular mechanisms of joint formation by transcription factor SOX11

转录因子SOX11形成关节的分子机制

• 批准号: 21591931
• 负责人: MATSUBARA Takehiro
• 金额: $ 3.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591931/
• 关键词: 関節病学; 永久関節軟骨再生

We analyzed the expression pattern of SOX11 in development of mouse embryo. Whole mount in situ hybridization revealed that Sox11 was expressed in the mesenchymal condensation and pre-mature chondrocytes in E11. 5-12. 5 embryo. Sox11 expression was maintained in the joint locus, and decreased as the joint matured. Next, we searched the transcriptional target genes of Sox11. Several screening revealed that Sox11 widely induced the cartilage matrix genes like Col2a1 and Acan, as well as the joint formation marker genes like GDF5 and Wnt9a. We further identified the responsive elements of Sox11 on these target genes. Meanwhile, Sox11 inhibited the expression of genes which were related with the endochondral ossification and the cartilage degradation. The molecular mechanisms underlying the inhibition were not elucidated yet.

我们分析了SOX11在小鼠胚胎发育过程中的表达模式。整体原位杂交显示Sox11在E11的间充质凝结细胞和早成熟软骨细胞中表达。 5-12。 5个胚胎。 Sox11 的表达在关节部位保持不变，并随着关节的成熟而减少。接下来，我们搜索了Sox11的转录靶基因。多项筛选表明，Sox11广泛诱导Col2a1和Acan等软骨基质基因，以及GDF5和Wnt9a等关节形成标记基因。我们进一步鉴定了 Sox11 对这些靶基因的响应元件。同时，Sox11抑制与软骨内骨化和软骨退化相关的基因的表达。抑制作用的分子机制尚未阐明。

项目成果

Combination of Two Urinary Biomarkers Predicts Acute Kidney Injury After Adult Cardiac Surgery

• DOI: 10.1016/j.athoracsur.2011.10.048
• 发表时间: 2012-02-01
• 期刊: ANNALS OF THORACIC SURGERY
• 影响因子: 4.6
• 作者: Katagiri, Daisuke;Doi, Kent;Noiri, Eisei
• 通讯作者: Noiri, Eisei

A Local Application of Recombinant Human Fibroblast Growth Factor 2 for Tibial Shaft Fractures: A Randomized, Placebo-Controlled Trial

• DOI: 10.1002/jbmr.146
• 发表时间: 2010-12-01
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Kawaguchi, Hiroshi;Oka, Hiroyuki;Nakamura, Kozo
• 通讯作者: Nakamura, Kozo

Biochemical markers of bone turnover as predictors of osteoporosis and osteoporotic fractures in men and women: 10-year follow-up of the Taiji cohort

• DOI: 10.3109/s10165-011-0455-2
• 发表时间: 2011-04
• 期刊: Modern Rheumatology
• 影响因子: 2.2
• 作者: N. Yoshimura;S. Muraki;H. Oka;H. Kawaguchi;Kozo Nakamura;T. Akune

Transcriptional induction of ADAMTS5 by NF-κB family member RelA/p65 in chondrocytes during osteoarthritis development

骨关节炎发展过程中软骨细胞中 NF-κB 家族成员 RelA/p65 转录诱导 ADAMTS5

• 发表时间: 2011
• 作者: Kobayashi H;Kawaguchi H;et al.
• 通讯作者: et al.

Notch/Rbpj/Hes1 Signal in Chondrocytes Modulates the Terminal Stage of Endochondral Ossification during Skeletal Growth and Osteoarthritis Development

软骨细胞中的 Notch/Rbpj/Hes1 信号调节骨骼生长和骨关节炎发展过程中软骨内骨化的末期

• 发表时间: 2011
• 作者: Hosaka Y;Saito T;et al.
• 通讯作者: et al.