Neuroprotective effects of combined estrogen and hypothermia after transient forebrain ischemia in rats

雌激素联合低温对大鼠短暂前脑缺血后的神经保护作用

• 批准号: 21591967
• 负责人: NAGASAKI Go
• 金额: $ 2.91万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591967/
• 关键词: 蘇生学; 一過性前脳虚血; 長期予後; エストロゲン; 低体温; ラット; 虚血性脳傷害

We examined whether combined treatment with estrogen and hypothermia reduces brain injury after transient forebrain ischemia in rats compared with either treatment alone.Methods : Male Sprague. Dawley rats were anesthetized with halothane and nitrous oxide in oxygen. Five groups were tested (n=8 each group): group C (vehicle 5 mg/kg, temporal muscle temperature 37.5℃); group E (17β-estradiol 200 μg/kg, 37.5℃); group H (vehicle 5 mg/kg, 35.0℃); group EH (17β-estradiol 200 μg/kg, 35.0℃); and group S (sham surgery, 37.5℃). In the groups H and EH, the temporal muscle temperature was reduced to 35℃ immediately after ischemia and maintained for 1 hour. Estrogen or vehicle was administered intravenously immediately after ischemia. Cerebral ischemia was produced by bilateral carotid artery occlusion with hemorrhagic hypotension (mean arterial pressure 40mmHg) for 10 min. Histopathology and neurologic deficit score were evaluated at 28 days after ischemia. Results : 28 days after ischemia, the numbers of intact neurons in the hippocampal CA1 subfield in the groups E (5±3/mm ; mean±SD), H (19±24/mm), EH (21±28/mm) were comparable with the group C(5±2/mm). The group S (132±22/mm)showed a significantly increased number of intact neurons compared with other four groups. There were no significant differences in neurologic deficit scores among five groups. Conclusion : A combination of estrogen and hypothermia did not provide greater neuroprotection compared with either treatment alone.

我们研究了雌激素和低温联合治疗是否能减少大鼠短暂性前脑缺血后的脑损伤，与单独治疗相比。道利大鼠用氟烷和一氧化二氮在氧气中麻醉。试验分为5组（每组n=8）：C组（溶媒5 mg/kg，颞肌温度37.5℃）; E组（17β-雌二醇200 μg/kg，37.5℃）; H组（溶媒5 mg/kg，35.0℃）; EH组（17β-雌二醇200 μg/kg，35.0℃）; S组（假手术，37.5℃）。H组和EH组于缺血后即刻将颞肌温度降至35℃并维持1h。缺血后立即静脉注射雌激素或溶媒。采用双侧颈总动脉夹闭加出血性低血压（平均动脉压40 mmHg）10 min造成脑缺血模型，缺血后28 d进行组织学和神经功能缺损评分。结果如下：缺血28天后，E组（5±3个/mm）、H组（19±24个/mm）、EH组（21±28个/mm）海马CA 1区完整神经元数与C组（5±2个/mm）相当。S组（132±22个/mm）较其他4组完整神经元数目明显增多。五组之间的神经功能缺损评分无显著差异。结论：雌激素和低温联合治疗与单独治疗相比，没有提供更好的神经保护作用。

项目成果

エストロゲン虚血後投与がラット海馬CA1細胞と短期記憶に及ぼす効果

缺血后雌激素给药对大鼠海马CA1细胞和短期记忆的影响

• 发表时间: 2010
• 作者: 長崎剛;戸部善継;西川俊昭
• 通讯作者: 西川俊昭

Long-term effects of post-treatment with estrogen on brain injury in a rat forebrain ischemia model

雌激素后处理对大鼠前脑缺血模型脑损伤的长期影响

• 发表时间: 2010
• 作者: Nagasaki G;Horiguchi T;Tobe Y;Nishikawa T
• 通讯作者: Nishikawa T