Analysis of volatile anestheics: involvement of intracellular signaling and neural transmission

挥发性麻醉药分析：细胞内信号传导和神经传递的参与

• 批准号: 21591988
• 负责人: NISHIMURA Kinya
• 金额: $ 2.75万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591988/
• 关键词: 吸入麻酔薬; 麻酔興奮期; tonicGABA電流; 吸入麻酔; 電気生理; 線状体; セボフルラン; GABA受容体; Tonic GABA電流; Patch clamp; 微小電流; テトロドトキシン; カドニウム; Caイオンチャネル; 神経回路; マウス; 線条体; EPSC; IPSC; 発火パターン

Suppression of movement during induction of anesthesia is mediated through subcortical structures. We studied the effects of a brief 5-min application of a clinically relevant concentration of sevoflurane on the electrophysiological activities of the medium spiny neurons of the stratum in brain slice preparations, using a whole-cell patch-clamp technique. We found that sevoflurane depressed the peak, as well as the net, charge transfer of intrastriatally evoked inhibitory postsynaptic currents much more strongly than those of excitatory postsynaptic currents. And this inhibition was accompanied by an elevated paired-pulse ratio. The strong suppression of eIPSCs paralleled a significant suppression of the spontaneous EPSCs. These results suggest that, in contrast to its effects on other brain structures, sevoflurane shifts the balance between synaptic excitation and inhibition in the direction of excitation in the stratum, thereby causing involuntary movements during induction of anesthesia by sevoflurane.And, Sevoflurane has been shown to enhance two types of GABAergic inhibition: a synaptic form (phasic inhibition) regulating neural excitability via the activation of postsynaptic GABAA receptors by intermittent GABA release from presynaptic terminals；and a persistent tonic form (tonic inhibition) generated by continuous activation of extrasynaptic GABAA receptors by low concentrations of ambient GABA.

麻醉诱导过程中运动的抑制是通过皮质下结构介导的。我们使用全细胞膜片钳技术研究了临床相关浓度的七氟醚短暂应用5分钟对脑切片制备中中层棘神经元电生理活动的影响。研究发现，七氟醚对脑内抑制性突触后电流峰和净电荷转移的抑制作用比兴奋性突触后电流强得多。这种抑制伴随着对脉比的升高。对EPSCs的强烈抑制与自发性EPSCs的显著抑制并行。这些结果表明，与七氟醚对其他脑结构的影响不同，七氟醚改变了突触兴奋和抑制之间的平衡，从而在七氟醚诱导麻醉过程中引起不自主运动。此外，七氟醚已被证明可以增强两种类型的GABA能抑制：一种是突触形式（相位抑制），通过突触前末端间歇性释放GABA激活突触后GABAA受体来调节神经兴奋性；另一种是持续的强直形式（强直抑制），通过低浓度的环境GABA持续激活突触外GABAA受体而产生。

项目成果

食道閉鎖症に対する胸腔鏡下吻合術に対する周術期管理

胸腔镜食管闭锁吻合术的围手术期处理

• 发表时间: 2009
• 作者: 工藤治;西村欣也;稲田英一;他;西村欣也
• 通讯作者: 西村欣也

Indications for thoracoscopic repair of congenital diaphragmatic hernia in neonates

胸腔镜新生儿先天性膈疝修补术的适应证

• DOI: 10.1007/s00383-010-2724-0
• 发表时间: 2010
• 期刊: Pediatric Surgery International
• 影响因子: 1.8
• 作者: T. Okazaki;Kinya Nishimura;Toshiaki Takahashi;H. Shoji;Toshiaki Shimizu;Toshitaka Tanaka;S. Takeda;E. Inada;Geoffrey J. Lane;A. Yamataka
• 通讯作者: A. Yamataka

脳内神経ネットワークからみた吸入麻酔薬の作用機序の解析

从脑内神经网络角度分析吸入麻醉药的作用机制

• 发表时间: 2012
• 期刊: 臨床麻酔
• 作者: 工藤治;西村欣也;稲田英一
• 通讯作者: 稲田英一

Thoracoscopic Plication for Diaphragmatic Eventration in a Neonate.

胸腔镜折叠术治疗新生儿膈肌膨出。

• 发表时间: 2012
• 期刊: Ann Thorac Cardiovasc Surg
• 作者: Oose Y，Miura M,;Inoue R;Andou，N,Aosaki T;Nishimura K;Takahashi T ,Nishimura K,
• 通讯作者: Takahashi T ,Nishimura K,

The benefit of stay sutures during thoracoscopic esophagoesophagostomy in patients with esophageal atresia: a technical report

• DOI: 10.1007/s00383-010-2560-2
• 发表时间: 2010-04-01
• 期刊: PEDIATRIC SURGERY INTERNATIONAL
• 影响因子: 1.8
• 作者: Shimotakahara, Akihiro;Sueyoshi, Ryo;Yamataka, Atsuyuki
• 通讯作者: Yamataka, Atsuyuki