Analgesic actions of nicotine for postoperative acute pain-Investigation by using in vivo patch-clamp recording.

我们建立了在体膜片钳技术的实验装置，用于记录大鼠脊髓背角神经元稳定的突触反应。脊髓表面灌流尼古丁剂量依赖性地增加大鼠脊髓背角神经元抑制性突触后电流的频率和幅度，提示尼古丁在脊髓中的镇痛机制之一。然而，静脉注射尼古丁对IPSC无明显影响。尼古丁对急性疼痛模型中兴奋性突触后电流、动作电位和反应的作用尚未确定。

We have arranged experimental facility for in vivo patch-clamp method to record stable synaptic responses in rat spinal dorsal horn neurons. Spinal surface perfusion of nicotine dose-dependently increased the frequency and amplitude of inhibitory post-synaptic currents in rat dorsal horn neurons of spinal cord suggesting one of the nicotine's analgesic mechanisms in the spinal cord. However, intravenous administration of nicotine had no obvious effects on IPSCs. Actions of nicotine on excitatory post-synaptic currents, action potentials, and responses in acute pain model are yet to be determined.