Analgesic actions of nicotine for postoperative acute pain-Investigation by using in vivo patch-clamp recording.

尼古丁对术后急性疼痛的镇痛作用——体内膜片钳记录研究。

• 批准号: 21592022
• 负责人: MORI Takashi
• 金额: $ 3万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592022/
• 关键词: 疼痛管理学; ニコチン; パッチクランプ法; 術後痛; 脊髄後角; in vivo; In vivo

We have arranged experimental facility for in vivo patch-clamp method to record stable synaptic responses in rat spinal dorsal horn neurons. Spinal surface perfusion of nicotine dose-dependently increased the frequency and amplitude of inhibitory post-synaptic currents in rat dorsal horn neurons of spinal cord suggesting one of the nicotine's analgesic mechanisms in the spinal cord. However, intravenous administration of nicotine had no obvious effects on IPSCs. Actions of nicotine on excitatory post-synaptic currents, action potentials, and responses in acute pain model are yet to be determined.

我们建立了在体膜片钳技术的实验装置，用于记录大鼠脊髓背角神经元稳定的突触反应。脊髓表面灌流尼古丁剂量依赖性地增加大鼠脊髓背角神经元抑制性突触后电流的频率和幅度，提示尼古丁在脊髓中的镇痛机制之一。然而，静脉注射尼古丁对IPSC无明显影响。尼古丁对急性疼痛模型中兴奋性突触后电流、动作电位和反应的作用尚未确定。

项目成果

気管挿管の確認法と食道挿管の鑑別

如何确认气管插管和区分食管插管

• 发表时间: 2009
• 期刊: 麻酔科学レクチャー 3
• 作者: 森隆;浅田章
• 通讯作者: 浅田章

全身麻酔薬がミクログリアP2X4受容体に及ぼす影響

全身麻醉药对小胶质细胞 P2X4 受体的影响

• 发表时间: 2010
• 作者: 羽阪めぐみ;森隆;松浦正;久野みゆき;西川精宣;浅田章
• 通讯作者: 浅田章

Systemic injection of alphα-2 adrenergic agonist enhances inhibitory synaptic transmission in the rat superficial dorsal horn in vivo

全身注射α-2肾上腺素能激动剂增强体内大鼠背浅角的抑制性突触传递

• 发表时间: 2011
• 作者: Yusuke Funai;Kiyonobu Nishikawa;Takashi Mori;Akira Asada;Keiji Imoto;Hidemasa Furue
• 通讯作者: Hidemasa Furue

Lidocaine modulation of voltage-gated proton channels in rat microglia.

利多卡因对大鼠小胶质细胞电压门控质子通道的调节。

• 发表时间: 2009
• 作者: Tadashi Matsuura;Takashi Mori;Miyuki Kuno;Megumi Hasaka;Kiyonobu Nishikawa;Makoto Sawada;Akira Asada
• 通讯作者: Akira Asada

Systemic injection of alpha-2 adrenergic agonist enhances inhibitory synaptic transmission in the rat superficial dorsal horn in vivo

全身注射α2肾上腺素能激动剂增强体内大鼠背浅角的抑制性突触传递

• 发表时间: 2011
• 作者: Funai Y;Nishikawa K;Mori T;Asada A;Imoto K;Furue H
• 通讯作者: Furue H